Anti-inflammatory response following uptake of apoptotic bodies by meningothelial cells
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4406167
Author(s) Li, Jia V.; Fang, Lei; Meyer, Peter; Killer, Hanspeter Esriel; Flammer, Josef; Neutzner, Albert
Author(s) at UniBasel Neutzner, Albert
Meyer, Peter
Year 2014
Title Anti-inflammatory response following uptake of apoptotic bodies by meningothelial cells
Journal Journal of neuroinflammation
Volume 11
Pages / Article-Number 35
Keywords Analysis of Variance; Animals; *Apoptosis; Cell Line, Tumor; Cytokines/*metabolism; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Humans; Imaging, Three-Dimensional; Meningioma/pathology; Microscopy, Confocal; Neuroblastoma/physiopathology; Phagocytes/*physiology; Swine
Mesh terms Analysis of Variance; Animals; Apoptosis; Cell Line, Tumor; Cytokines, metabolism; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Humans; Imaging, Three-Dimensional; Meningioma, pathology; Microscopy, Confocal; Neuroblastoma, physiopathology; Phagocytes, physiology; Swine
Abstract BACKGROUND: Meningothelial cells (MECs) are the cellular components of the meninges. As such, they provide important barrier function for the central nervous system (CNS) building the interface between neuronal tissue and the cerebrospinal fluid (CSF), and are also part of the immune response of the CNS. METHODS: Human, immortalized MECs were analyzed by flow cytometry and confocal microscopy to study the uptake of apoptotic cells. Furthermore, cytokine and chemokine production by MECs was analyzed by cytokine array and ELISA. RESULTS: We found that MECs are highly active phagocytes able of ingesting and digesting large amounts of apoptotic cells. Furthermore, the uptake of apoptotic cells by MECs was immune suppressive via inhibiting the secretion of pro-inflammatory and chemoattractant cytokines and chemokines IL-6, IL-8, IL-16, MIF, and CXCL1, while increasing the secretion of anti-inflammatory IL-1 receptor antagonist by MECs. CONCLUSION: MECs respond with the secretion of anti-inflammatory cytokines and chemokines following the uptake of apoptotic cells potentially connecting these cells to processes important for the shut-down of immune responses in the brain.
Publisher BioMed Central
ISSN/ISBN 1742-2094
URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3941933/
edoc-URL https://edoc.unibas.ch/62366/
Full Text on edoc No
Digital Object Identifier DOI 10.1186/1742-2094-11-35
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/24565420
ISI-Number WOS:000333240100002
Document type (ISI) Journal Article
 
   

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