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Immunoregulatory effects of the flavonol quercetin in vitro and in vivo
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 4376361
Author(s) Nickel, Thomas; Hanssen, Henner; Sisic, Zeljka; Pfeiler, Susanne; Summo, Claudia; Schmauss, Daniel; Hoster, Eva; Weis, Michael
Author(s) at UniBasel Hanssen, Henner
Year 2011
Title Immunoregulatory effects of the flavonol quercetin in vitro and in vivo
Journal European Journal of Nutrition
Volume 50
Number 3
Pages / Article-Number 163-72
Mesh terms Adult; Animals; Anti-Inflammatory Agents, pharmacology; Apoptosis; Arginine, blood; Cell Adhesion; Cell Differentiation; Cell Line; Dendritic Cells, metabolism; Endocytosis; Endothelial Cells, metabolism; Humans; Leukocytes, metabolism; Lipoproteins, LDL, metabolism; Male; Mice; Mice, Inbred C57BL; Monocytes, immunology; NF-kappa B, drug effects; Quercetin, pharmacology; Up-Regulation
Abstract Atherosclerosis is known to be an inflammatory disease. Dendritic cells (DCs) are essential for the regulation of the immune system. Up to 10% of the cells in atherosclerotic plaques are DCs. The cardiovascular protective effects of flavonoids (tea, wine) may be mediated by anti-inflammatory mechanisms that affect DC regulation. We aimed to characterize the impact of the flavonol quercetin on DC activity and differentiation in vitro and in vivo.; For the in vitro experiments, we used murine DCs and endothelial cells to study adhesion properties. For all other experiments (DC phagocytosis capacity, DC maturation, DC differentiation (BDCA-1/-2) and NF-kB-activation), human monocyte-derived DCs were used. The cells were incubated with quercetin (10 μmol/L) ± oxLDL (10 μg/mL) between 24 and 48 h. For in vivo experiments, eight healthy male volunteers took 500 mg of quercetin twice daily over 4 weeks, five healthy male volunteers served as control. Before and after intake, blood samples were collected. Peripheral blood leukocytes were isolated (analyses of DC differentiation), and plasma was immediately frozen.; Quercetin reduced DC adhesion (-42%; p < 0.05) and expression of CD11a (-21%; p < 0.05). OxLDL-induced DC differentiation was partially inhibited by quercetin (BDCA-1-29%; BDCA-2-33%; p < 0.05). These effects were achieved by compensation of oxLDL-induced up-regulation of NF-kB by quercetin. The 4-week treatment with quercetin resulted in relevant plasma levels (2.47 μmol/L) and reduced BDCA-2 + DCs in the peripheral blood by 42% (p < 0.05) as well as systemic levels of the NO-synthase inhibitor asymmetric dimethylarginine (-31%, p < 0.05).; In vitro, quercetin reduced DC adhesion and oxLDL-induced DC differentiation. In vivo, quercetin reduced circulating plasmacytoid DCs and systemic ADMA-levels. The immunoregulatory effects of quercetin may contribute to the anti-atherosclerotic potential of flavonols.
Publisher Springer
ISSN/ISBN 1436-6215
edoc-URL https://edoc.unibas.ch/62125/
Full Text on edoc No
Digital Object Identifier DOI 10.1007/s00394-010-0125-8
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/20652710
ISI-Number 000289581900002
Document type (ISI) Journal Article
 
   

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