Data Entry: Please note that the research database will be replaced by UNIverse by the end of October 2023. Please enter your data into the system https://universe-intern.unibas.ch. Thanks

Login for users with Unibas email account...

Login for registered users without Unibas email account...

 
Target-directed Dynamic Combinatorial Chemistry: A Study on Potentials and Pitfalls as Exemplified on a Bacterial Target
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 3886720
Author(s) Frei, Priska; Pang, Lijuan; Silbermann, Marleen; Eriş, Deniz; Mühlethaler, Tobias; Schwardt, Oliver; Ernst, Beat
Author(s) at UniBasel Ernst, Beat
Frei, Priska
Pang, Lijuan
Silbermann, Marleen
Eris, Deniz
Mühlethaler, Tobias
Schwardt, Oliver
Year 2017
Title Target-directed Dynamic Combinatorial Chemistry: A Study on Potentials and Pitfalls as Exemplified on a Bacterial Target
Journal Chemistry - A European Journal
Volume 23
Number 48
Pages / Article-Number 11570-11577
Abstract Target-directed dynamic combinatorial chemistry (DCC) is an emerging technique for the efficient identification of inhibitors of pharmacologically relevant targets. In this contribution, we present an application for a bacterial target, the lectin FimH, a crucial virulence factor of uropathogenic E. coli being the main cause of urinary tract infections. A small dynamic library of acylhydrazones was formed from aldehydes and hydrazides and equilibrated at neutral pH in presence of aniline as nucleophilic catalyst. The major success factors turned out to be an accordingly adjusted ratio of scaffolds and fragments, an adequate sample preparation prior to HPLC analysis, and the data processing. Only then did the ranking of the dynamic library constituents correlate well with affinity data. Furthermore, as a support of DCC applications especially to larger libraries, a new protocol for improved hit identification was established.
Publisher Wiley
ISSN/ISBN 0947-6539 ; 1521-3765
edoc-URL http://edoc.unibas.ch/55826/
Full Text on edoc No
Digital Object Identifier DOI 10.1002/chem.201701601
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/28654733
ISI-Number WOS:000408308200018
Document type (ISI) Journal Article
 
   

MCSS v5.8 PRO. 0.345 sec, queries - 0.000 sec ©Universität Basel  |  Impressum   |    
29/03/2024