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BIOINSPIRED FUNCTIONAL PROTEIN-POLYMER SUPRAMOLECULAR NANOASSEMBLIES
Third-party funded project
Project title BIOINSPIRED FUNCTIONAL PROTEIN-POLYMER SUPRAMOLECULAR NANOASSEMBLIES
Principal Investigator(s) Palivan, Cornelia
Project Members Zartner, Luisa
Organisation / Research unit Departement Chemie / Physikalische Chemie (Palivan)
Project start 01.07.2017
Probable end 30.06.2021
Status Completed
Abstract

The development of new functional materials by bottom-up approaches that combine different building blocks at the nanoscale in a novel architecture with improved or new properties and functionality is currently in focus for applications in various domains. One of the most promising strategies is to interface biomolecules (enzymes, proteins, DNA, biomimics) with synthetic assemblies that have a variety of architectures (micelles, vesicles, tubes, particles) in order to mimic natural structures and functions. Such bio-hybrid materials have the advantages of combining the activity and specificity of biomolecules with the stability and precise topology of a synthetic matrix, and thus underscore conventional systems in terms of efficacy and functionality. This project aims to develop functional protein-polymer assemblies, which will serve as new types of catalytic nanocompartments providing efficient and controlled reaction space for biomolecules by combining physical chemistry, nanoscience and enzyme biochemistry. In a biomimetic approach inspired by natural organelles in living cells, we will encapsulate/insert biomolecules (proteins, enzymes) in synthetic nanocompartments to create catalytic reaction spaces with different topology and functionality. We will produce catalytic nanocompartments with “triggered activity” so that in situ reactions inside compartments are activated by opening “protein gates” inserted inside the membrane. A complementary objective is based on producing catalytic nanocompartments acting “in tandem” to support cascade reactions involving a combination of different nanocompartments. Catalytic nanocompartments, producing desired molecules “on demand” serve as simple mimics of natural organelles, whereas those acting “in tandem” mimic chemical communication between organelles. The development of enzymatic reactions in the confined spaces of supramolecular assemblies with nanometer sizes represents a response to the increasing evidence of low bioavailability and stability of directly administrated biopharmaceuticals, and the necessity to produce/detect compounds by controlled cascade reactions between different spatial assemblies in new materials with complex functionality (electronics, medicine, catalysis, food science). The understanding at the molecular level of the relationships between the factors that support successful reactions in confined spaces with different topologies represents a major benefit of this project, which aims to combine model enzymatic reactions with complementary properties resulting from different architectures of the reaction spaces (single-, and in tandem- nanocompartments) Systematic variation of the nanocompartment properties (size, thickness of the membrane, concentration of compartments), and characteristics of the biomolecules (concentration, modification with specific molecular entities, ratio between different biomolecules) will produce efficient interfacing and result in new functional hybrid assemblies. The project combines a fundamental study of the structural changes and interactions that occur when a functional bio-hybrid assembly is generated with applied investigations of the relevant factors and conditions that characterise “model” enzymatic reactions for extension to other reactions necessary for translational applications. This will support the development of a rational design for an efficient platform of catalytic nanocompartments by optimizing the structural and functional details for each type of reaction space (single-, and in tandem- nanocompartments), and straightforward changes of biomolecule or the overall polymer assembly.

Keywords Nano-compartiments polymériques; organelles artificielles; réactions en cascade; enzymes; protéines membranaires
Financed by Swiss National Science Foundation (SNSF)

Published results ()

  ID Autor(en) Titel ISSN / ISBN Erschienen in Art der Publikation
4634566  Zartner, Luisa; Maffeis, Viviana; Schoenenberger, Cora-Ann; Dinu, Ionel Adrian; Palivan, Cornelia G.  Membrane protein channels equipped with a cleavable linker for inducing catalysis inside nanocompartments  2050-750X ; 2050-7518  Journal of Materials Chemistry B  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
4607687  Zartner, Luisa; Garni, Martina; Craciun, Ioana; Einfalt, Tomaz; Palivan, Cornelia G.  How Can Giant Plasma Membrane Vesicles Serve as a Cellular Model for Controlled Transfer of Nanoparticles?  1525-7797 ; 1526-4602  Biomacromolecules  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
4600045  Zartner, Luisa; Muthwill, Moritz S.; Dinu, Ionel Adrian; Schoenenberger, Cora-Ann; Palivan, Cornelia G.  The rise of bio-inspired polymer compartments responding to pathology-related signals  2050-750X ; 2050-7518  Journal of Materials Chemistry B  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
4527274  Einfalt, Tomaz; Garni, Martina; Witzigmann, Dominik; Sieber, Sandro; Baltisberger, Niklaus; Huwyler, Jörg; Meier, Wolfgang; Palivan, Cornelia  Bioinspired Molecular Factories with Architecture and In Vivo Functionalities as Cell Mimics  2198-3844  Advanced Science  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
4599261  Meyer, Claire E.; Abram, Sarah-Luise; Craciun, Ioana; Palivan, Cornelia G.  Biomolecule-polymer hybrid compartments: combining the best of both worlds  1463-9076 ; 1463-9084  Physical chemistry chemical physics  JournalItem (Kommentare, Editorials, Rezensionen, Urteilsanmerk., etc. in einer wissensch. Zeitschr. 
4527299  Belluati, Andrea; Mikhalevich, Viktoria; Yorulmaz Avsar, Saziye; Daubian, Davy; Craciun, Ioana; Chami, Mohamed; Meier, Wolfgang P.; Palivan, Cornelia G.  How Do the Properties of Amphiphilic Polymer Membranes Influence the Functional Insertion of Peptide Pores?  1525-7797 ; 1526-4602  Biomacromolecules  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
4527286  Yorulmaz Avsar, Saziye; Kyropoulou, Myrto; Di Leone, Stefano; Schoenenberger, Cora-Ann; Meier, Wolfgang P.; Palivan, Cornelia G.  Biomolecules Turn Self-Assembling Amphiphilic Block Co-polymer Platforms Into Biomimetic Interfaces  2296-2646  Frontiers in Chemistry  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
4527281  Garni, M.; Wehr, R.; Yorulmaz Avsar, S.; John, C.; Palivan, C.G.; Meier, W.  Polymer membranes as templates for bio-applications ranging from artificial cells to active surfaces  0014-3057  European polymer journal  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
4527248  Thamboo, Sagana; Najer, Adrian; Belluati, Andrea; von Planta, Claudio; Wu, Dalin; Craciun, Ioana; Meier, Wolfgang; Palivan, Cornelia G.  Mimicking Cellular Signaling Pathways within Synthetic Multicompartment Vesicles with Triggered Enzyme Activity and Induced Ion Channel Recruitment    Advanced Functional Materials  Publication: JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift) 
   

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