Anion–π Enzymes
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 3728436
Author(s) Cotelle, Yoann; Lebrun, Vincent; Sakai, Naomi; Ward, Thomas R.; Matile, Stefan
Author(s) at UniBasel Lebrun, Vincent
Ward, Thomas R.
Matile, Stefan
Year 2016
Year: comment 2016
Title Anion–π Enzymes
Journal ACS Central Science
Volume 2
Number 6
Pages / Article-Number 388-393
Abstract

In this report, we introduce artificial enzymes that operate with anion-π interactions, an interaction that is essentially new to nature. The possibility to stabilize anionic intermediates and transition states on an π-acidic surface has been recently demonstrated, using the addition of malonate half thioesters to enolate acceptors as a biologically relevant example. The best chiral anion-π catalysts operate with an addition/decarboxylation ratio of 4:1, but without any stereoselectivity. To catalyze this important but intrinsically disfavored reaction stereoselectively, a series of anion-π catalysts was equipped with biotin and screened against a collection of streptavidin mutants. With the best hit, the S112Y mutant, the reaction occurred with 95% ee and complete suppression of the intrinsically favored side product from decarboxylation. This performance of anion-π enzymes rivals, if not exceeds, that of the best conventional organocatalysts. Inhibition of the S112Y mutant by nitrate but not by bulky anions supports that contributions from anion-π interactions exist and matter, also within proteins. In agreement with docking results, K121 is shown to be essential, presumably to lower the p K a of the tertiary amine catalyst to operate at the optimum pH around 3, that is below the p K a of the substrate. Most importantly, increasing enantioselectivity with different mutants always coincides with increasing rates and conversion, i.e., selective transition-state stabilization.

Publisher American Chemical Society
ISSN/ISBN 2374-7943 ; 2374-7951
edoc-URL http://edoc.unibas.ch/53910/
Full Text on edoc Available
Digital Object Identifier DOI 10.1021/acscentsci.6b00097
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/27413782
ISI-Number WOS:000378690000007
Document type (ISI) Article
 
   

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