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GintAMT3 - a Low-Affinity Ammonium Transporter of the Arbuscular Mycorrhizal Rhizophagus irregularis
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 3676126
Author(s) Calabrese, Silvia; Pérez-Tienda, Jacob; Ellerbeck, Matthias; Arnould, Christine; Chatagnier, Odile; Boller, Thomas; Schüßler, Arthur; Brachmann, Andreas; Wipf, Daniel; Ferrol, Nuria; Courty, Pierre-Emmanuel
Author(s) at UniBasel Boller, Thomas
Year 2016
Title GintAMT3 - a Low-Affinity Ammonium Transporter of the Arbuscular Mycorrhizal Rhizophagus irregularis
Journal Frontiers in Plant Science
Volume 7
Pages / Article-Number 679
Abstract Nutrient acquisition and transfer are essential steps in the arbuscular mycorrhizal (AM) symbiosis, which is formed by the majority of land plants. Mineral nutrients are taken up by AM fungi from the soil and transferred to the plant partner. Within the cortical plant root cells the fungal hyphae form tree-like structures (arbuscules) where the nutrients are released to the plant-fungal interface, i.e., to the periarbuscular space, before being taken up by the plant. In exchange, the AM fungi receive carbohydrates from the plant host. Besides the well-studied uptake of phosphorus (P), the uptake and transfer of nitrogen (N) plays a crucial role in this mutualistic interaction. In the AM fungus Rhizophagus irregularis (formerly called Glomus intraradices), two ammonium transporters (AMT) were previously described, namely GintAMT1 and GintAMT2. Here, we report the identification and characterization of a newly identified R. irregularis AMT, GintAMT3. Phylogenetic analyses revealed high sequence similarity to previously identified AM fungal AMTs and a clear separation from other fungal AMTs. Topological analysis indicated GintAMT3 to be a membrane bound pore forming protein, and GFP tagging showed it to be highly expressed in the intraradical mycelium of a fully established AM symbiosis. Expression of GintAMT3 in yeast successfully complemented the yeast AMT triple deletion mutant (MATa ura3 mep1Δ mep2Δ::LEU2 mep3Δ::KanMX2). GintAMT3 is characterized as a low affinity transport system with an apparent Km of 1.8 mM and a V max of 240 nmol(-1) min(-1) 10(8) cells(-1), which is regulated by substrate concentration and carbon supply.
Publisher Frontiers Media
ISSN/ISBN 1664-462X
edoc-URL http://edoc.unibas.ch/45192/
Full Text on edoc No
Digital Object Identifier DOI 10.3389/fpls.2016.00679
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/27252708
ISI-Number WOS:000376744300001
Document type (ISI) Journal Article
 
   

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