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Antibody-Mediated Neutralization of the Exotoxin Mycolactone, the Main Virulence Factor Produced by Mycobacterium ulcerans
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 3644821
Author(s) Dangy, Jean-Pierre; Scherr, Nicole; Gersbach, Philipp; Hug, Melanie N; Bieri, Raphael; Bomio, Claudio; Li, Jun; Huber, Sylwia; Altmann, Karl-Heinz; Pluschke, Gerd
Author(s) at UniBasel Pluschke, Gerd
Scherr, Nicole
Year 2016
Title Antibody-Mediated Neutralization of the Exotoxin Mycolactone, the Main Virulence Factor Produced by Mycobacterium ulcerans
Journal PLoS Neglected Tropical Diseases
Volume 10
Number 6
Pages / Article-Number e0004808
Abstract Mycolactone, the macrolide exotoxin produced by Mycobacterium ulcerans, causes extensive tissue destruction by inducing apoptosis of host cells. In this study, we aimed at the production of antibodies that could neutralize the cytotoxic activities of mycolactone.; Using the B cell hybridoma technology, we generated a series of monoclonal antibodies with specificity for mycolactone from spleen cells of mice immunized with the protein conjugate of a truncated synthetic mycolactone derivative. L929 fibroblasts were used as a model system to investigate whether these antibodies can inhibit the biological effects of mycolactone. By measuring the metabolic activity of the fibroblasts, we found that anti-mycolactone mAbs can completely neutralize the cytotoxic activity of mycolactone.; The toxin neutralizing capacity of anti-mycolactone mAbs supports the concept of evaluating the macrolide toxin as vaccine target.
Publisher Public Library of Science
ISSN/ISBN 1935-2727 ; 1935-2735
edoc-URL http://edoc.unibas.ch/44515/
Full Text on edoc Available
Digital Object Identifier DOI 10.1371/journal.pntd.0004808
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/27351976
ISI-Number WOS:000379346200053
Document type (ISI) Journal Article
 
   

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