Analytical interference of 4-hydroxy-3-methoxymethamphetamine with the measurement of plasma free normetanephrine by ultra-high pressure liquid chromatography-tandem mass spectrometry
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 3454147
Author(s) Dunand, Marielle; Donzelli, Massimiliano; Rickli, Anna; Hysek, Cédric M.; Liechti, Matthias E.; Grouzmann, Eric
Author(s) at UniBasel Liechti, Matthias Emanuel
Year 2014
Title Analytical interference of 4-hydroxy-3-methoxymethamphetamine with the measurement of plasma free normetanephrine by ultra-high pressure liquid chromatography-tandem mass spectrometry
Journal Clinical biochemistry
Volume 47
Number 12
Pages / Article-Number 1121-3
Mesh terms Chromatography, High Pressure Liquid, methods; Healthy Volunteers; Humans; Methamphetamine, blood; Normetanephrine, blood; Tandem Mass Spectrometry, methods
Abstract The diagnosis of pheochromocytoma relies on the measurement of plasma free metanephrines assay whose reliability has been considerably improved by ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Here we report an analytical interference occurring between 4-hydroxy-3-methoxymethamphetamine (HMMA), a metabolite of 3,4-methylenedioxymethamphetamine (MDMA, "Ecstasy"), and normetanephrine (NMN) since they share a common pharmacophore resulting in the same product ion after fragmentation.; Synthetic HMMA was spiked into plasma samples containing various concentrations of NMN and the intensity of the interference was determined by UPLC-MS/MS before and after improvement of the analytical method.; Using a careful adjustment of chromatographic conditions including the change of the UPLC analytical column, we were able to distinguish both compounds. HMMA interference for NMN determination should be seriously considered since MDMA activates the sympathetic nervous system and if confounded with NMN may lead to false-positive tests when performing a differential diagnostic of pheochromocytoma.
Publisher Elsevier
ISSN/ISBN 0009-9120 ; 1873-2933
edoc-URL https://edoc.unibas.ch/62031/
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.clinbiochem.2014.04.003
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/24731790
ISI-Number 000340995900027
Document type (ISI) Journal Article
 
   

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