In the large collaborative project NCCR RNA&Disease, funded by SNF, we have two main sub-projects. In the first project we explore the function of an isoform of Argonaute 1 protein, which is generated by translation readthough, beyond the canonical stop codon. This isoform is extremely conserved in evolution, and we have indications that it carries out an entirely distinct function relative to other human mammalian Argonautes, promoting proliferation of cancer cells. The second sub-project follows our computational inference that a particular RNA binding protein, the polypyrimidine tract binding protein 1, affects the processing of RNAs in a particular cancer, glioblastoma. In this project we aim to characterize the mechanism and targets of this protein, as well as the contribution of specific targets in glioblastoma development.