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Antibody and T-cell responses associated with experimental human malaria infection or vaccination show limited relationships
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 3122666
Author(s) Walker, Karen M; Okitsu, Shinji; Porter, David W; Duncan, Christopher; Amacker, Mario; Pluschke, Gerd; Cavanagh, David R; Hill, Adrian V S; Todryk, Stephen M
Author(s) at UniBasel Pluschke, Gerd
Year 2015
Title Antibody and T-cell responses associated with experimental human malaria infection or vaccination show limited relationships
Journal Immunology
Volume 145
Number 1
Pages / Article-Number 71-81
Keywords antibodies, infectious diseases, malaria, T cells
Abstract

This study examined specific antibody and T-cell responses associated with experimental malaria infection or malaria vaccination, in malaria-naive human volunteers within phase I/IIa vaccine trials, with a view to investigating inter-relationships between these types of response. Malaria infection was via five bites of Plasmodium falciparum-infected mosquitoes, with individuals reaching patent infection by 11-12 days, having harboured four or five blood-stage cycles before drug clearance. Infection elicited a robust antibody response against merozoite surface protein-119 , correlating with parasite load. Classical class switching was seen from an early IgM to an IgG1-dominant response of increasing affinity. Malaria-specific T-cell responses were detected in the form of interferon-γ and interleukin-4 (IL-4) ELIspot, but their magnitude did not correlate with the magnitude of antibody or its avidity, or with parasite load. Different individuals who were immunized with a virosome vaccine comprising influenza antigens combined with P. falciparum antigens, demonstrated pre-existing interferon-γ, IL-2 and IL-5 ELIspot responses against the influenza antigens, and showed boosting of anti-influenza T-cell responses only for IL-5. The large IgG1-dominated anti-parasite responses showed limited correlation with T-cell responses for magnitude or avidity, both parameters being only negatively correlated for IL-5 secretion versus anti-apical membrane antigen-1 antibody titres. Overall, these findings suggest that cognate T-cell responses across a range of magnitudes contribute towards driving potentially effective antibody responses in infection-induced and vaccine-induced immunity against malaria, and their existence during immunization is beneficial, but magnitudes are mostly not inter-related.

Publisher Blackwell
ISSN/ISBN 0019-2805
edoc-URL http://edoc.unibas.ch/dok/A6381873
Full Text on edoc No
Digital Object Identifier DOI 10.1111/imm.12428
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/25471322
ISI-Number WOS:000353060000007
Document type (ISI) Journal Article, Multicenter Study
 
   

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