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The complete mitochondrial genome of the invasive Ponto-Caspian goby Ponticola kessleri obtained from high-throughput sequencing using the Ion Torrent Personal Genome Machine
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
The complete mitochondrial genome of the invasive Ponto-Caspian goby Ponticola kessleri obtained from high-throughput sequencing using the Ion Torrent Personal Genome Machine
We report the first complete mitochondrial genome (mitogenome) of an invasive Ponto-Caspian goby, Ponticola kessleri (bighead goby, Gu¨ nther 1891). Ion Torrent PGM sequencing oftotal DNA from two individuals yielded a contig of 16,971 bp, with overlapping ends located inthe repetitive control region, which was validated using Sanger sequencing. The finalmitogenome of Ponticola kessleri has a size of 16,890 bp and contains the expected geneconfiguration of 13 protein-coding genes, 2 rRNA genes and 22 tRNA genes. In a comparisonwith complete mitogenomes from other goby species, we identified a translocation of tRNAGluin the mitogenome of P. kessleri. Rearrangements are unique and rare events, and can thus providephylogenetic information.
