Data Entry: Please note that the research database will be replaced by UNIverse by the end of October 2023. Please enter your data into the system https://universe-intern.unibas.ch. Thanks

Login for users with Unibas email account...

Login for registered users without Unibas email account...

 
A resource for the conditional ablation of microRNAs in the mouse
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 2612340
Author(s) Park, Chong Yon; Jeker, Lukas T.; Carver-Moore, Karen; Oh, Alyssia; Liu, Huey Jiin; Cameron, Rachel; Richards, Hunter; Li, Zhongmei; Adler, David; Yoshinaga, Yuko; Martinez, Maria; Nefadov, Michael; Abbas, Abul K.; Weiss, Art; Lanier, Lewis L.; de Jong, Pieter J.; Bluestone, Jeffrey A.; Srivastava, Deepak; McManus, Michael T.
Author(s) at UniBasel Jeker, Lukas
Year 2012
Title A resource for the conditional ablation of microRNAs in the mouse
Journal Cell Reports
Volume 1
Number 4
Pages / Article-Number 385-391
Abstract The importance of miRNAs during development and disease processes is well established. However, most studies have been done in cells or with patient tissues, and therefore the physiological roles of miRNAs are not well understood. To unravel in vivo functions of miRNAs, we have generated conditional, reporter-tagged knockout-first mice for numerous evolutionarily conserved miRNAs. Here, we report the generation of 162 miRNA targeting vectors, 64 targeted ES cell lines, and 46 germline-transmitted miRNA knockout mice. In vivo lacZ reporter analysis in 18 lines revealed highly tissue-specific expression patterns and their miRNA expression profiling matched closely with published expression data. Most miRNA knockout mice tested were viable, supporting a mechanism by which miRNAs act redundantly with other miRNAs or other pathways. These data and collection of resources will be of value for the in vivo dissection of miRNA functions in mouse models.
Publisher Elsevier
ISSN/ISBN 2211-1247
URL http://www.ncbi.nlm.nih.gov/pmc/articles/pmc3345170/
edoc-URL https://edoc.unibas.ch/61962/
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.celrep.2012.02.008
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/22570807
ISI-Number 000309712000011
Document type (ISI) Article
 
   

MCSS v5.8 PRO. 0.345 sec, queries - 0.000 sec ©Universität Basel  |  Impressum   |    
28/03/2024