Absorbance detector based on a deep UV light emitting diode for narrow-column HPLC
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 2310248
Author(s) Bui, Duy Anh; Bomastyk, Benjamin; Hauser, Peter C
Author(s) at UniBasel Hauser, Peter C.
Year 2013
Title Absorbance detector based on a deep UV light emitting diode for narrow-column HPLC
Journal Journal of separation science
Volume 36
Number 19
Pages / Article-Number 3152-7
Keywords Light-emitting diode, Narrow-bore chromatography, UV detection

A detector for miniaturized HPLC based on deep UV emitting diodes and UV photodiodes was constructed. The measurement is accomplished by the transverse passage of the radiation from the light-emitting diode (LED) through fused-silica tubing with an internal diameter of 250 m. The optical cell allows flexible alignment of the LED, tubing, and photodiode for optimization of the light throughput and has an aperture to block stray light. A beam splitter was employed to direct part of the emitted light to a reference photodiode and the Lambert-Beer law was emulated with a log-ratio amplifier circuitry. The detector was tested with two LEDs with emission bands at 280 and 255 nm and showed noise levels as low as 0.25 and 0.22 mAU, respectively. The photometric device was employed successfully in separations using a column of 1 mm inner diameter in isocratic as well as gradient elution. Good linearities over three orders of magnitude in concentration were achieved, and the precision of the measurements was better than 1% in all cases. Detection down to the low micromolar range was possible.

Publisher Wiley-VCH
ISSN/ISBN 1615-9314
edoc-URL http://edoc.unibas.ch/dok/A6212113
Full Text on edoc No
Digital Object Identifier DOI 10.1002/jssc.201300598
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/23893947
ISI-Number WOS:000325139700002
Document type (ISI) Article

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