A genetically encoded calcium indicator for chronic in vivo two-photon imaging
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 2250687
Author(s) Mank, Marco; Santos, Alexandre Ferrão; Direnberger, Stephan; Mrsic-Flogel, Thomas D.; Hofer, Sonja B.; Stein, Valentin; Hendel, Thomas; Reiff, Dierk F.; Levelt, Christiaan; Borst, Alexander; Bonhoeffer, Tobias; Hübener, Mark; Griesbeck, Oliver
Author(s) at UniBasel Hofer, Sonja
Mrsic-Flogel, Thomas
Year 2008
Title A genetically encoded calcium indicator for chronic in vivo two-photon imaging
Journal Nature Methods
Volume 5
Number 9
Pages / Article-Number 805-811
Abstract Neurons in the nervous system can change their functional properties over time. At present, there are no techniques that allow reliable monitoring of changes within identified neurons over repeated experimental sessions. We increased the signal strength of troponin C-based calcium biosensors in the low-calcium regime by mutagenesis and domain rearrangement within the troponin C calcium binding moiety to generate the indicator TN-XXL. Using in vivo two-photon ratiometric imaging, we show that TN-XXL exhibits enhanced fluorescence changes in neurons of flies and mice. TN-XXL could be used to obtain tuning curves of orientation-selective neurons in mouse visual cortex measured repeatedly over days and weeks. Thus, the genetically encoded calcium indicator TN-XXL allows repeated imaging of response properties from individual, identified neurons in vivo, which will be crucial for gaining new insights into cellular mechanisms of plasticity, regeneration and disease.
Publisher Nature Publishing Group
ISSN/ISBN 1548-7091 ; 1548-7105
edoc-URL http://edoc.unibas.ch/49618/
Full Text on edoc No
Digital Object Identifier DOI 10.1038/nmeth.1243
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/19160515
ISI-Number WOS:000258912700014
Document type (ISI) Article

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