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Mechanical nanosensor based on FRET within a thermosome : damage-reporting polymeric materials
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 221261
Author(s) Bruns, Nico; Pustelny, Katarzyna; Bergeron, Lisa M; Whitehead, Timothy A; Clark, Douglas S
Author(s) at UniBasel Bruns, Nico
Year 2009
Title Mechanical nanosensor based on FRET within a thermosome : damage-reporting polymeric materials
Journal Angewandte Chemie
Volume 48
Number 31
Pages / Article-Number 5666-9
Keywords FRET, hybrid materials, polymers, proteins, stress detection
Abstract

Under stress: Changes in stress of the polymer matrix in a protein-polymer hybrid material result in changes of conformation of the protein complex, thus resulting in a damage-reporting material (see picture). The reporter is an engineered chaperonin that covalently entraps a pair of fluorescent proteins. Deformation of the chaperonin leads to a change in fluorophore distance and a change in the fluorescene resonance energy transfer (FRET) signal.

Publisher Wiley-VCH
ISSN/ISBN 0044-8249
URL http://www3.interscience.wiley.com/journal/122368586/abstract
edoc-URL http://edoc.unibas.ch/dok/A5265392
Full Text on edoc No
Digital Object Identifier DOI 10.1002/anie.200900554
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/19402088
ISI-Number WOS:000268538100016
Document type (ISI) Journal Article
Additional Information Also published in: Angewandte Chemie. International edition in English. - Weinheim : VCH. - Vol. 48, H. 31 (2009), S. 5666–5669
 
   

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