Bacterial Type IV Secretion (T4S): Cellular, Molecular, and Evolutionary Basis of the Subversion of Host Cell Functions by Translocated Effector Proteins
Third-party funded project
Project title Bacterial Type IV Secretion (T4S): Cellular, Molecular, and Evolutionary Basis of the Subversion of Host Cell Functions by Translocated Effector Proteins
Principal Investigator(s) Dehio, Christoph
Project Members Stanger, Frédéric
Siamer, Sabrina
Ben Tekaya, Houchaima
Organisation / Research unit Departement Biozentrum / Molecular Microbiology (Dehio)
Project start 01.10.2013
Probable end 30.09.2016
Status Completed
Abstract

In the initial funding period of the SNSF grant 31003A-132979 we used Bartonella and Brucella – two related zoonotic pathogens engaging the widespread type IV secretion (T4S) mechanism for establishing chronic bacterial infection - as models to study the cellular, molecular and evolutionary basis of the subversion of host cell functions by T4S-translocated effector proteins. For the three year prolongation period the established multidisciplinary experimental approach will be extended by adopting yeast as surrogate model for studying conserved eukaryotic processes targeted by T4S effectors. Subproject A will focus on the structure/function analysis of Bartonella effector proteins (Beps) translocated by the VirB T4S system of Bartonella and their physiological consequences on the host, with particular emphasis on studying the subversion of immune signaling processes. Subproject B will focus on the functional analysis of effector proteins translocated by the distinct VirB T4S system of Brucella and their physiological consequences on host cell interaction, with particular emphasis on studying the intracellular trafficking events from a late endosomal compartment, where the VirB system is activated in response to acidification, to the endoplasmic reticulum (ER)-related replicative niche of Brucella. Together, these studies will contribute to establishing a molecular paradigm for the role of T4S effectors in triggering chronic bacterial infection.

Keywords type IV secretion (T4S), Bartonella effector proteins
Financed by Swiss National Science Foundation (SNSF)

Cooperations ()

  ID Kreditinhaber Kooperationspartner Institution Laufzeit - von Laufzeit - bis
2291861  Dehio, Christoph  Schirmer, Tilman, Professor  Biozentrum, University of Basel  01.10.2013  30.09.2016 
   

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