Bacteria as a versatile tool for single protein delivery into eukaryotic cells

This CTI feasibility project introduces a bacteria-based tool that allows to translocate eukaryotic proteins into various eukaryotic cells using an effector-less Yersinia enterocolitica strain. The method provides fast, synchronized and tuneable protein translocation which allows functional interaction studies by the injection of multiple proteins simultaneously. Further, the protein delivery can be easily upscaled und  thus be coupled to –omics studies and was shown to work in vivo.