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The anticipation of danger: microbe-associated molecular pattern perception enhances AtPep-triggered oxidative burst
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1939135
Author(s) Flury, Pascale; Klauser, Dominik; Schulze, Birgit; Boller, Thomas; Bartels, Sebastian
Author(s) at UniBasel Boller, Thomas
Klauser, Dominik
Merker, Sebastian
Year 2013
Title The anticipation of danger: microbe-associated molecular pattern perception enhances AtPep-triggered oxidative burst
Journal Plant physiology
Volume 161
Number 4
Pages / Article-Number 2023-35
Abstract

The endogenous Arabidopsis (Arabidopsis thaliana) peptides, AtPeps, elicit an innate immune response reminiscent of pattern-triggered immunity. Detection of various danger signals, including microbe-associated molecular patterns (MAMPs), leads to elevated transcription of PROPEPs, the AtPep precursors, and PEPRs, the AtPep receptors. It has been hypothesized that AtPeps are involved in enhancing pattern-triggered immunity. Following this idea, we analyzed the relationship between MAMP- and AtPep-elicited signaling. We found that the perception of MAMPs enhanced a subsequent AtPep-triggered production of reactive oxygen species (ROS). Intriguingly, other components of AtPep-triggered immunity like Ca(2+) influx, mitogen-activated protein kinase phosphorylation, ethylene production, and expression of early defense genes, as well as ROS-activated genes, remained unchanged. By contrast, treatment with methyl jasmonate promoted an increase of all analyzed AtPep-triggered responses. We positively correlated the intensities of generic AtPep-triggered responses with the abundance of the two AtPep receptors by generating constitutively expressing PEPR1 and PEPR2 transgenic lines and by analyzing pepr1 and pepr2 mutants. Further, we show that enhanced, as well as basal, ROS production triggered by AtPeps is absent in the double mutant of the respiratory burst oxidase homologs D and F (rbohD rbohF). We present evidence that the enhancement of AtPep-triggered ROS is not based on changes in the ROS detoxification machinery and is independent of mitogen-activated protein kinase and Ca(2+) signaling pathways. Taken together, these results indicate an additional level of regulation besides receptor abundance for the RbohD/RbohF-dependent production of AtPep-elicited ROS, which is specifically operated by MAMP-triggered pathways.

Publisher American Society of Plant Biologists
ISSN/ISBN 0032-0889
edoc-URL http://edoc.unibas.ch/dok/A6164896
Full Text on edoc No
Digital Object Identifier DOI 10.1104/pp.113.216077
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/23400703
ISI-Number WOS:000316987900034
Document type (ISI) Journal Article
 
   

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23/02/2024