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The family of ammonium transporters (AMT) in Sorghum bicolor : two AMT members are induced locally, but not systemically in roots colonized by arbuscular mycorrhizal fungi
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1939134
Author(s) Koegel, Sally; Ait Lahmidi, Nassima; Arnould, Christine; Chatagnier, Odile; Walder, Florian; Ineichen, Kurt; Boller, Thomas; Wipf, Daniel; Wiemken, Andres; Courty, Pierre-Emmanuel
Author(s) at UniBasel Koegel, Sally
Wiemken, Andres M.
Courty, Pierre-Emmanuel
Boller, Thomas
Year 2013
Title The family of ammonium transporters (AMT) in Sorghum bicolor : two AMT members are induced locally, but not systemically in roots colonized by arbuscular mycorrhizal fungi
Journal The new phytologist
Volume 198
Number 3
Pages / Article-Number 853-65
Keywords ammonium and phosphate transporters, arbuscular mycorrhiza, immunolocalization, microdissection, nitrogen, sorghum (Sorghum bicolor)
Abstract

Arbuscular mycorrhizal (AM) fungi contribute to plant nitrogen (N) acquisition. Recent studies demonstrated the transport of N in the form of ammonium during AM symbiosis. Here, we hypothesize that induction of specific ammonium transporter (AMT) genes in Sorghum bicolor during AM colonization might play a key role in the functionality of the symbiosis. For the first time, combining a split-root experiment and microdissection technology, we were able to assess the precise expression pattern of two AM-inducible AMTs, SbAMT3;1 and SbAMT4. Immunolocalization was used to localize the protein of SbAMT3;1. The expression of SbAMT3;1 and SbAMT4 was greatly induced locally in root cells containing arbuscules and in adjacent cells. However, a split-root experiment revealed that this induction was not systemic. By contrast, a strictly AM-induced phosphate transporter (SbPt11) was expressed systemically in the split-root experiment. However, a gradient of expression was apparent. Immunolocalization analyses demonstrated that SbAMT3;1 was present only in cells containing developing arbuscules. Our results show that the SbAMT3;1 and SbAMT4 genes are expressed in root cortical cells, which makes them ready to accommodate arbuscules, a process of considerable importance in view of the short life span of arbuscules. Additionally, SbAMT3;1 might play an important role in N transfer during AM symbiosis.

Publisher Blackwell Science
ISSN/ISBN 0028-646X
edoc-URL http://edoc.unibas.ch/dok/A6164895
Full Text on edoc No
Digital Object Identifier DOI 10.1111/nph.12199
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/23461653
ISI-Number WOS:000317682900021
Document type (ISI) Journal Article
 
   

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