Antiprotozoal activity of Khaya anthotheca, (Welv.) C.D.C. A plant used by chimpanzees for self medication
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1777738
Author(s) Obbo, C. J. D.; Makanga, B.; Mulholland, D. A.; Coombes, P. H.; Brun, R.
Author(s) at UniBasel Brun, Reto
Year 2013
Title Antiprotozoal activity of Khaya anthotheca, (Welv.) C.D.C. A plant used by chimpanzees for self medication
Journal Journal of ethnopharmacology : an interdisciplinary journal devoted to bioscientific research on indigenous drugs
Volume 147
Number 1
Pages / Article-Number 220-223
Keywords Khaya anthotheca, Meliaceae, Chimpanzees, Self-medication, Antiprotozoal, Drug-resistance
Abstract

ETHNOPHARMACOLOGICAL RELEVANCE: Khaya species, endemic to Africa and Madagascar, continues to be valuable in indigenous traditional medicine. Their bitter tasting barks are decocted to treat fevers, several febrile conditions, microbial infections and worm infestations. In the Budongo rain forest of Western Uganda, non-human primates, especially chimpanzees and baboons, have been observed to eat the bitter non-nutritious bark and occasionally the seed. MATERIALS AND METHODS: Extracts were prepared by sequential fractionation with solvents of increasing polarities and assayed using standard procedures. Bioassay guided purification of the petroleum ether extract by column chromatography yielded three pure limonoids, Grandifolione (1), 7-deacetylkhivorin (2) and 1,3-deacetyldeoxyhavenensin (3). The antitrypanosomal, antileishmanial and antiplasmodial activities of pure compounds (1) and (2) were evaluated in vitro against Plasmodium falciparum K1, Trypanosoma brucei rhodesiense STIB 900, Trypanosoma cruzi trypomastigotes (Tulahuen C4), and axenic Leishmania donovani MHOMET-67/L82 and for cytotoxicity against L6 rat skeletal myoblast cells, in parallel with standard drugs. RESULTS: Of the four extracts tested, the petroleum ether extract showed activity against Plasmodium falciparum (IC50 0.955?g/ml) and Trypanosoma brucei rhodesiense (IC50 5.72?g/ml). The pure compounds (1) and (2) demonstrated activity against Plasmodium falciparum (KI strain) and marginal activities against Trypanosoma brucei brucei, Trypanosoma brucei rhodesiense, Trypanosoma cruzi and Leishmania donovani. CONCLUSION: The present study provides evidence justifying the use of Khaya preparations in traditional medicine to treat fevers and microbial infections. The observed antiprotozoal activity of grandifolione and 7-deacetylkhivorin from the seed of Khaya anthotheca further confirms the ethnomedicinal potential of this plant and supports the hypothesis that non-human hominids (chimpanzees and baboons) too, eat the bitter bark and seeds for self-medication and in general, the use of Khaya plant material for medication by humans in disease endemic tropical areas. The antiprotozoal activity of gradifolione, and, the antitrypanosomal and antileishmanial activities of 7-deacetylkhivorin are reported here for the first time.

Publisher Elsevier Scientific Publishers
ISSN/ISBN 0378-8741
URL http://www.ncbi.nlm.nih.gov/pubmed/23501156
edoc-URL http://edoc.unibas.ch/dok/A6124612
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.jep.2013.03.007
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/23501156
ISI-Number WOS:000318386000022
Document type (ISI) Article
 
   

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