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A new rapid diagnostic test for detection of anti-Schistosoma mansoni and anti-Schistosoma haematobium antibodies
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1765003
Author(s) Coulibaly, Jean T.; N'Goran, Eliezer K.; Utzinger, Juerg; Doenhoff, Michael J.; Dawson, Emily M.
Author(s) at UniBasel Utzinger, Jürg
Year 2013
Title A new rapid diagnostic test for detection of anti-Schistosoma mansoni and anti-Schistosoma haematobium antibodies
Journal Parasites and Vectors
Volume 6
Number 29
Keywords Schistosomiasis, Schistosoma haematobium, Schistosoma mansoni, Diagnosis, Antibodies, Cercariae, Sensitivity, Specificity, Positive predictive value, Negative predictive value, Cote d'Ivoire
Abstract Parasitological methods are widely used for the diagnosis of schistosomiasis. However, they are insensitive, particularly in areas of low endemicity, and labour-intensive. Immunoassays based on detection of anti-schistosome antibodies have the merit of high sensitivity and recently a rapid diagnostic test (RDT), incorporating Schistosoma mansoni cercarial transformation fluid (SmCTF) for detection of anti-schistosome antibodies in blood has been developed. Here, we assessed the diagnostic performance of the SmCTF-RDT for S. mansoni and S. haematobium infections by comparing it with microscopy for egg detection.; A cross-sectional survey was carried out in Azaguié, south Côte d'Ivoire. 118 pre-school-aged children submitted two stool and two urine samples, which were subjected to the Kato-Katz and urine filtration methods for the detection of S. mansoni and S. haematobium eggs, respectively. Urine was also subjected to a commercially available cassette test for S. mansoni, which detects circulating cathodic antigen. A finger-prick blood sample was used for the SmCTF-RDT for detection of anti-S. mansoni and anti-S. haematobium antibodies.; The prevalence of both anti-S. mansoni and anti-S. haematobium antibodies was more than three times higher than the prevalence of infection estimated by egg detection under a microscope. Using quadruplicate Kato-Katz as the reference standard for the diagnosis of S. mansoni infection, the sensitivity, negative predictive value (NPV), and positive predictive value (PPV) of the SmCTF-RDT was 75.0%, 84.2% and 22.5%, respectively. When two urine filtrations were considered as the reference standard for the diagnosis of S. haematobium infection, the sensitivity, NPV and PPV of SmCTF-RDT was 66.7%, 94.9% and 5.1%, respectively. The specificity of SmCTF-RDT, when using egg-detection as the reference standard, was estimated to be 34.4%. This low specificity may be a reflection of the relative insensitivity of the direct diagnostic approaches using microscopy.; The SmCTF-RDT is at least as sensitive as duplicate Kato-Katz and a single urine filtration for detection of S. mansoni and S. haematobium, respectively. Further investigations into the specificity of the test for anti-schistosome antibodies are necessary, but our results suggest that it may be a useful tool for mapping the prevalence of anti-schistosome antibodies in a given population pending intervention.
Publisher BioMed Central
ISSN/ISBN 1756-3305
edoc-URL http://edoc.unibas.ch/dok/A6124536
Full Text on edoc Available
Digital Object Identifier DOI 10.1186/1756-3305-6-29
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/23360734
ISI-Number WOS:000315455600001
Document type (ISI) Article
 
   

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29/03/2024