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The contrast-imaging function for tilted specimens
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 175709
Author(s) Philippsen, Ansgar; Engel, Hans-Andreas; Engel, Andreas
Author(s) at UniBasel Engel, Andreas
Year 2007
Title The contrast-imaging function for tilted specimens
Journal Ultramicroscopy
Volume 107
Number 2-3
Pages / Article-Number 202-212
Keywords CTF, space-variant point spread function, 3D reconstruction
Abstract

A theoretical description of the contrast-imaging function is derived for tilted specimens that exhibit weak-phase object characteristics. We show that the tilted contrast-imaging function (TCIF) is a linear transformation, which can be approximated by the convolution operation for small tilt angles or for small specimens. This approximation is not valid for electron tomography, where specimen tilts are above 60 degrees and specimen dimensions amount to some 10mum. The approximation also breaks down for electron crystallography, where atomic resolution is to be achieved. Therefore, we do not make this approximation and propose a generalized algorithm for inverting the TCIF. The implications of our description are discussed in the context of electron tomography, single particle analysis, and electron crystallography, and the improved resolution is quantitatively demonstrated.

Publisher Elsevier
ISSN/ISBN 0304-3991
URL http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16989948
edoc-URL http://edoc.unibas.ch/dok/A5262434
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.ultramic.2006.07.010, 10.1016/j.ultramic.2006.07.010
ISI-Number WOS:000243710800016
Document type (ISI) Article
 
   

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