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Are small GTPases signal hubs in sugar-mediated induction of fructan biosynthesis?
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 169621
Author(s) Ritsema, T.; Brodmann, D.; Diks, S. H.; Bos, C. L.; Nagaraj, V.; Pieterse, C. M. J.; Boller, T.; Wiemken, A.; Peppelenbosch, M. P.
Author(s) at UniBasel Boller, Thomas
Wiemken, Andres M.
Year 2009
Title Are small GTPases signal hubs in sugar-mediated induction of fructan biosynthesis?
Journal PLoS ONE
Volume 4
Number 8
Keywords fructan biosynthesis, protein phosphorylation, enzyme regulation
Abstract External sugar initiates biosynthesis of the reserve carbohydrate fructan, but the molecular processes mediating this response remain obscure. Previously it was shown that a phosphatase and a general kinase inhibitor hamper fructan accumulation. We use various phosphorylation inhibitors both in barley and in Arabidopsis and show that the expression of fructan biosynthetic genes is dependent on PP2A and different kinases such as Tyr-kinases and PI3-kinases. To further characterize the phosphorylation events involved, comprehensive analysis of kinase activities in the cell was performed using a PepChip, an array of <1000 kinase consensus substrate peptide substrates spotted on a chip. Comparison of kinase activities in sugar-stimulated and mock(sorbitol)-treated Arabidopsis demonstrates the altered phosphorylation of many consensus substrates and documents the differences in plant kinase activity upon sucrose feeding. The different phosphorylation profiles obtained are consistent with sugar-mediated alterations in Tyr phosphorylation, cell cycling, and phosphoinositide signaling, and indicate cytoskeletal rearrangements. The results lead us to infer a central role for small GTPases in sugar signaling.
Publisher Public Library of Science
ISSN/ISBN 1932-6203
edoc-URL http://edoc.unibas.ch/dok/A5261892
Full Text on edoc No
Digital Object Identifier DOI 10.1371/journal.pone.0006605
ISI-Number WOS:000268935900016
Document type (ISI) Article
 
   

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21/04/2024