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Autodisplay : efficacious surface exposure of antigenic UreA fragments from Helicobacter pylori in Salmonella vaccine strains
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 156868
Author(s) Rizos, K.; Lattemann, C. T.; Bumann, D.; Meyer, T. F.; Aebischer, T.
Author(s) at UniBasel Bumann, Dirk
Year 2003
Title Autodisplay : efficacious surface exposure of antigenic UreA fragments from Helicobacter pylori in Salmonella vaccine strains
Journal Infection and Immunity
Volume 71
Number 11
Pages / Article-Number 6320-8
Keywords Adhesins; Escherichia coli/immunology; Animals; Bacterial Vaccines/*immunology; Female; Helicobacter pylori/*immunology; Immunization; Mice; Inbred BALB C; Peptide Fragments/*immunology; Recombinant Fusion Proteins/immunology; Salmonella/*genetics; Urease/*immunology; Vaccines; Synthetic/*immunology
Abstract Live attenuated Salmonella strains expressing antigens of pathogens are promising oral vaccine candidates. There is growing evidence that the topology of expression of the foreign antigens can have a dramatic impact on the immunogenicity. We examined the potential of the AIDA-I (Escherichia coli adhesin involved in diffuse adherence) autotransporter domain to display antigenic fragments of the urease A subunit of Helicobacter pylori for the induction of a protective immune response. In the murine H. pylori model, protection is mainly mediated by CD4(+) T cells, and we therefore used the AIDA-I expression system to successfully express both nearly full-length UreA and defined T-helper-cell epitopes on the surface of an attenuated Salmonella enterica serovar Typhimurium vaccine strain. Surface exposure of the large UreA fragment or of one UreA T-cell epitope mediated a significant reduction in the level of H. pylori in immunized mice after challenge infection, whereas conventional cytoplasmic expression of UreA in Salmonella had no effect. These results support the concept that surface display increases the immunogenicity of recombinant antigens expressed on oral live vaccine carriers and further demonstrate the feasibility of immunizing against H. pylori with Salmonella vaccine strains expressing CD4(+) T-cell epitopes.
Publisher American Society for Microbiology
ISSN/ISBN 0019-9567 ; 1098-5522
edoc-URL http://edoc.unibas.ch/dok/A5259817
Full Text on edoc No
Digital Object Identifier DOI 10.1128/IAI.71.11.6320-6328.2003
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/14573651
ISI-Number WOS:000186194300027
Document type (ISI) Journal Article
 
   

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