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Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 156791
Author(s) Trautwein, M.; Schindler, C.; Gauss, R.; Dengjel, J.; Hartmann, E.; Spang, A.
Author(s) at UniBasel Spang, Anne
Year 2006
Title Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi
Journal The EMBO Journal
Volume 25
Number 5
Pages / Article-Number 943-54
Keywords arf1, ChAPs, coat, golgi, vesicular transport
Abstract In Saccharomyces cerevisiae, the synthesis of chitin is temporally and spatially regulated through the transport of Chs3p (chitin synthase III) to the plasma membrane in the bud neck region. Traffic of Chs3p from the trans-Golgi network (TGN)/early endosome to the plasma membrane requires the function of Chs5p and Chs6p. Chs6p belongs to a family of four proteins that we have named ChAPs for Chs5p-Arf1p-binding Proteins. Here, we show that all ChAPs physically interact not only with Chs5p but also with the small GTPase Arf1p. A short sequence at the C-terminus of the ChAPs is required for protein function and the ability to bind to Chs5p. Simultaneous disruption of two members, Deltabud7 and Deltabch1, phenocopies a Deltachs6 or Deltachs5 deletion with respect to Chs3p transport. Moreover, the ChAPs interact with each other and can form complexes. In addition, they are all at least partially localized to the TGN in a Chs5p-dependent manner. Most importantly, several ChAPs can interact physically with Chs3p. We propose that the ChAPs facilitate export of cargo out of the Golgi.
Publisher Nature Publishing Group
ISSN/ISBN 0261-4189 ; 1460-2075
edoc-URL http://edoc.unibas.ch/dok/A5259744
Full Text on edoc No
Digital Object Identifier DOI 10.1038/sj.emboj.7601007
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/16498409
ISI-Number WOS:000236225400003
Document type (ISI) Journal Article
 
   

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