A mammalian microRNA expression atlas based on small RNA library sequencing
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 156697
Author(s) Landgraf, P.; Rusu, M.; Sheridan, R.; Sewer, A.; Iovino, N.; Aravin, A.; Pfeffer, S.; Rice, A.; Kamphorst, A. O.; Landthaler, M.; Lin, C.; Socci, N. D.; Hermida, L.; Fulci, V.; Chiaretti, S.; Foa, R.; Schliwka, J.; Fuchs, U.; Novosel, A.; Muller, R. -U.; Schermer, B.; Bissels, U.; Inman, J.; Phan, Q.; Chien, M.; Weir, D. B.; Choksi, R.; De Vita, G.; Frezzetti, D.; Trompeter, H. -I.; Hornung, V.; Teng, G.; Hartmann, G.; Palkovits, M.; Di Lauro, R.; Wernet, P.; Macino, G.; Rogler, C. E.; Nagle, J. W.; Ju, J.; Papavasiliou, F. N.; Benzing, T.; Lichter, P.; Tam, W.; Brownstein, M. J.; Bosio, A.; Borkhardt, A.; Russo, J. J.; Sander, C.; Zavolan, M.; Tuschl, T.
Author(s) at UniBasel Zavolan, Mihaela
Year 2007
Title A mammalian microRNA expression atlas based on small RNA library sequencing
Journal Cell
Volume 129
Number 7
Pages / Article-Number 1401-1414
Keywords Animals; Base Sequence/*genetics; Cell Lineage/genetics; Conserved Sequence/genetics; Gene Expression Profiling/*methods; Gene Expression Regulation/*genetics; *Gene Library; Hematologic Neoplasms/genetics; Hematopoietic Stem Cells/metabolism; Humans; Mice; MicroRNAs/*genetics; Molecular Sequence Data; Phylogeny; RNA; Messenger/genetics; Rats; Sequence Homology; Nucleic Acid
Abstract MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses.
Publisher Cell Press
ISSN/ISBN 0092-8674
URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2681231/
edoc-URL http://edoc.unibas.ch/dok/A5259654
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.cell.2007.04.040
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/17604727
ISI-Number 000247911400024
Document type (ISI) Article

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