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N-terminal sequence of pig brain choline acetyltransferase purified by a rapid procedure
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 156605
Author(s) Braun, A; Barde, Y A; Lottspeich, F; Mewes, W; Thoenen, H
Author(s) at UniBasel Barde, Yves-Alain
Year 1987
Title N-terminal sequence of pig brain choline acetyltransferase purified by a rapid procedure
Journal Journal of Neurochemistry
Volume 48
Number 1
Pages / Article-Number 16-21
Keywords Amino Acid Sequence; Amino Acids/analysis; Animals; Brain/*enzymology; Choline O-Acetyltransferase/*isolation & purification; Chromatography; Affinity; Electrophoresis; *Peptide Fragments; Swine
Abstract A procedure is reported that allows the purification and amino terminal sequencing of pig brain choline acetyltransferase. The enzyme (present in extremely low amounts in this tissue) is eluted together with its antibody from an affinity column by a mild pH shift and the resulting enzyme-antibody complex separated by gel electrophoresis. The band corresponding to the enzyme is electroeluted from the gel using volatile solutions allowing the direct determination of the amino acid composition and partial sequence. The first 11 residues are: Pro-Ile-Leu-Glu-Lys-Thr-Pro-Pro-Lys-Met-Ala.
Publisher Wiley-Blackwell
ISSN/ISBN 0022-3042
edoc-URL http://edoc.unibas.ch/dok/A5259571
Full Text on edoc No
Digital Object Identifier DOI 10.1111/j.1471-4159.1987.tb13121.x
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/3794697
ISI-Number WOS:A1987F351600003
Document type (ISI) Article
 
   

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