A crucial role for neurotrophin-3 in oligodendrocyte development
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 156578
Author(s) Barres, B A; Raff, M C; Gaese, F; Bartke, I; Dechant, G; Barde, Y A
Author(s) at UniBasel Barde, Yves-Alain
Year 1994
Title A crucial role for neurotrophin-3 in oligodendrocyte development
Journal Nature
Volume 367
Number 6461
Pages / Article-Number 371-5
Keywords Animals; Astrocytes/drug effects/metabolism; Brain-Derived Neurotrophic Factor; Cell Differentiation; Cell Division; Cells; Cultured; Culture Media; DNA/biosynthesis; Nerve Growth Factors/metabolism/pharmacology/*physiology; Nerve Tissue Proteins/pharmacology; Neurotrophin 3; Oligodendroglia/*cytology; Optic Nerve/cytology; Platelet-Derived Growth Factor/pharmacology; Rats; Receptor Protein-Tyrosine Kinases/biosynthesis/genetics; Receptor; trkC; Receptors; Growth Factor/biosynthesis/genetics; Stem Cells/*cytology
Abstract The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4/5 promote the survival of subpopulations of vertebrate neurons in vitro, but so far only nerve growth factor has been demonstrated to be essential for normal neuronal development; no neurotrophin has previously been shown to function in normal glial cell development. We found recently that neurotrophin-3 promotes the survival of pure oligodendrocyte precursor cells in vitro, and, although by itself it induces only a small percentage of these cells to synthesize DNA, in combination with platelet-derived growth factor it induces the majority of them to do so. Neither of these factors, however, has been shown to contribute to oligodendrocyte precursor cell proliferation in vivo or to stimulate pure populations of these cells to proliferate (as opposed to synthesize DNA) in vitro. Here we show that neurotrophin-3 and platelet-derived growth factor collaborate to promote clonal expansion of oligodendrocyte precursor cells in vitro and to drive the intrinsic clock that times oligodendrocyte development. We also show that neurotrophin-3 helps stimulate the proliferation of oligodendrocyte precursor cells in vivo and is thus required for normal oligodendrocyte development.
Publisher Macmillan
ISSN/ISBN 0028-0836
edoc-URL http://edoc.unibas.ch/dok/A5259544
Full Text on edoc No
Digital Object Identifier DOI 10.1038/367371a0
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/8114937
ISI-Number WOS:A1994MT54000068
Document type (ISI) Journal Article

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