A restriction map of virulence plasmid pVYE439-80 from a serogroup 9 Yersinia enterocolitica strain
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 156287
Author(s) Laroche, Y; van Bouchaute, M; Cornelis, G
Author(s) at UniBasel Cornelis, Guy R.
Year 1984
Title A restriction map of virulence plasmid pVYE439-80 from a serogroup 9 Yersinia enterocolitica strain
Journal Plasmid
Volume 12
Number 1
Pages / Article-Number 67-70
Keywords Base Sequence; DNA Restriction Enzymes/diagnostic use; *Plasmids; Serotyping; Yersinia enterocolitica/classification/*genetics
Abstract A restriction map of the virulence plasmid pVYE439-80, isolated from Yersinia enterocolitica 439-80 (serogroup 9) was constructed for EcoRI, BamHI, SstII, and SmaI. The mapping was done after cloning of about two-thirds of the plasmid in Escherichia coli. The restriction pattern was compared to those obtained with plasmids isolated from Y. enterocolitica strains of serogroups 1, 3, and 5b. The restriction sites are particularly conserved in a region of about 25 kb. This region contains fragments that are also conserved in serogroup 8 strains [J. Heeseman, C. Keller, R. Morawa, N. Schmidt, H. J. Siemens, and R. Lauf (1983) J. Infect. Dis. 147, 107-115] and that were shown, in strains from this serogroup, to encode calcium dependency [D. A. Portnoy, H. Wolf-Watz, I. Bolin, A. B. Beeder, and S. Falkow, (1984) Infect. Immun. 43, 108-114].
Publisher Academic Press
ISSN/ISBN 0147-619X
edoc-URL http://edoc.unibas.ch/dok/A5259272
Full Text on edoc No
Digital Object Identifier DOI 10.1016/0147-619X(84)90069-6
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/6093172
ISI-Number WOS:A1984TH41600009
Document type (ISI) Journal Article
 
   

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