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A new method for the physical and genetic mapping of large plasmids : application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 156281
Author(s) Michiels, T; Popoff, M Y; Durviaux, S; Coynault, C; Cornelis, G
Author(s) at UniBasel Cornelis, Guy R.
Year 1987
Title A new method for the physical and genetic mapping of large plasmids : application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium
Journal Microbial pathogenesis
Volume 3
Number 2
Pages / Article-Number 109-16
Keywords DNA Mutational Analysis; DNA Transposable Elements; *Genes; Bacterial; *Plasmids; Replicon; *Restriction Mapping; Salmonella typhimurium/*genetics/pathogenicity
Abstract A new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.
Publisher Academic Press
ISSN/ISBN 0882-4010
edoc-URL http://edoc.unibas.ch/dok/A5259266
Full Text on edoc No
Digital Object Identifier DOI 10.1016/0882-4010(87)90069-6
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/2849015
ISI-Number WOS:A1987J682900005
Document type (ISI) Journal Article
 
   

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