AMPA receptors regulate dynamic equilibrium of presynaptic terminals in mature hippocampal networks
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 156098
Author(s) De Paola, Vincenzo; Arber, Silvia; Caroni, Pico
Author(s) at UniBasel Arber, Silvia
Year 2003
Title AMPA receptors regulate dynamic equilibrium of presynaptic terminals in mature hippocampal networks
Journal Nature neuroscience
Volume 6
Number 5
Pages / Article-Number 491-500
Keywords Animals; Hippocampus/cytology/drug effects/*physiology; Mice; Transgenic; Nerve Net/cytology/drug effects/*physiology; Presynaptic Terminals/drug effects/*physiology; Receptors; AMPA/agonists/antagonists & inhibitors/*physiology
Abstract The formation and disassembly of synapses in mature neuropil could provide a substrate to encode experience in the brain. Although there is evidence for postsynaptic spine dynamics in mature systems, contributions to circuit rearrangements by presynaptic terminals have remained unclear. We used hippocampal slice cultures from mice expressing spectral variants of green fluorescent protein (GFP) that are targeted to the membrane and/or synaptic vesicles in neuronal subsets to image identified presynaptic terminals. In mature tissues with no net change in synapse numbers, subpopulations of presynaptic terminals appeared and disappeared within 1-3 days. The three terminal types established by mossy fibers had distinct properties. High-frequency stimulation increased the fraction of dynamic terminals for 1-2 days, a process mediated by activation of AMPA receptors, protein kinase A (PKA) and protein synthesis. Thus, synaptic activity can make stable presynaptic terminals become dynamic, providing a candidate mechanism to convert experience into changes in network connectivity.
Publisher Nature America
ISSN/ISBN 1097-6256
edoc-URL http://edoc.unibas.ch/dok/A5259085
Full Text on edoc No
Digital Object Identifier DOI 10.1038/nn1046
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/12692557
ISI-Number WOS:000182553200016
Document type (ISI) Journal Article
 
   

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