An engineered heparin-binding form of VEGF-E (hbVEGF-E) : biological effects in vitro and mobilizatiion of precursor cells
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 156022
Author(s) Heil, Matthias; Mitnacht-Krauss, Rita; Issbrücker, Katja; van den Heuvel, Joop; Dehio, Christoph; Schaper, Wolfgang; Clauss, Matthias; Weich, Herbert A
Author(s) at UniBasel Dehio, Christoph
Year 2003
Title An engineered heparin-binding form of VEGF-E (hbVEGF-E) : biological effects in vitro and mobilizatiion of precursor cells
Journal Angiogenesis
Volume 6
Number 3
Pages / Article-Number 201-11
Keywords Binding Sites; Bone Marrow Cells/drug effects/physiology; Cell Movement/drug effects; Cell Proliferation/drug effects; Endothelium; Vascular/cytology/drug effects; Hematopoietic Stem Cell Mobilization; Heparin/metabolism; Humans; Neovascularization; Physiologic/drug effects; Protein Binding; *Protein Engineering; Recombinant Fusion Proteins/genetics/*pharmacology; Vascular Endothelial Growth Factor A/genetics/pharmacology; Vascular Endothelial Growth Factors/*genetics/pharmacology; Viral Proteins/genetics/pharmacology
Abstract Vascular endothelial growth factor (VEGF-A) is the founding member of a family of angiogenic proteins with various binding abilities to three cognate VEGF receptors. Previously, a gene encoding from the genome of parapox orf virus (OV) with about 25% amino acid identity to mammalian VEGF-A was named VEGF-E and shown to bind and specifically activate the vascular endothelial growth factor receptor VEGFR-2 (KDR/flk-1). Here, we have generated a novel heparin-binding form of VEGF-E by introducing the heparin-domain of the human VEGF-A(165) splice variant into the viral VEGF-E protein. Recombinant heparin-binding VEGF-E (hbVEGF-E) is shown to stimulate proliferation and sprout formation of macro- and microvascular endothelial cells to a similar extent as the parental OV-VEGF-E but fails to activate peripheral mononuclear cells. However, hbVEGF-E is more potent in binding competition assays with primary human endothelial cells when compared to the OV-VEGF-E. This can be explained by our finding that binding of hbVEGF-E but not of parental OV-VEGF-E to the VEGFR-2 is strongly increased by the addition of neuropilin-1 (NP-1), a cognate co-receptor for VEGF-A. The engineered hbVEGF-E was compared with the VEGFR-1 selective and also heparin-binding form of placenta growth factor (PlGF-2) in vivo. Both heparin-binding homologues induced mobilization of endothelial progenitor cells from the bone marrow and gave rise to similar colony numbers of myeloic cells in a colony-forming assay. These findings suggest that both VEGFR-1 and VEGFR-2 are involved in stem cell mobilization.
Publisher Kluwer
ISSN/ISBN 0969-6970
edoc-URL http://edoc.unibas.ch/dok/A5259014
Full Text on edoc No
Digital Object Identifier DOI 10.1023/B:AGEN.0000021391.88601.92
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/15041796
ISI-Number BCI:BCI200400338556
Document type (ISI) Journal Article
 
   

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