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Audio-Frequency NMR in a Nutating Frame : application to the Assignment of Phenylalanine Residues in Isotopically Enriched Proteins
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 155831
Author(s) GRZESIEK, S; BAX, A
Author(s) at UniBasel Grzesiek, Stephan
Year 1995
Title Audio-Frequency NMR in a Nutating Frame : application to the Assignment of Phenylalanine Residues in Isotopically Enriched Proteins
Journal Journal of the American Chemical Society
Volume 117
Number 24
Pages / Article-Number 6527-6531
Abstract The effect of chemical shift can be removed to high order by quantizing nuclear spins along a strong radio-frequency (RF) field and applying a second audio-frequency magnetic field, orthogonal to the direction of the effective RF spin lock field. By adjusting the audio-modulation frequency of this second magnetic field to the rate at which spins nutate about the RF field, a resonance condition in the spin-locked frame is established which, to second order, is independent of the resonance offset of the homonuclear spins. The phase and amplitude of the audio-frequency field are under operator control and make it possible to conduct complex pulse sequences in the nutating frame. The approach is demonstrated for transferring in a single step magnetization from phenylalanine C-13(beta) to all aromatic ring carbons in a sample of uniformly C-13-enriched calmodulin. A three-dimensional version of the experiment yields complete resonance assignments for the structurally informative aromatic Phe signals, including all C-13(zeta) and H-1(zeta) resonances.
Publisher AMER CHEMICAL SOC
ISSN/ISBN 0002-7863
edoc-URL http://edoc.unibas.ch/dok/A5258833
Full Text on edoc No
Digital Object Identifier DOI 10.1021/ja00129a016
ISI-Number WOS:A1995RE57500016
Document type (ISI) Article
 
   

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