Agrin is highly expressed by chondrocytes and is required for normal growth
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 155345
Author(s) Hausser, Heinz-Juergen; Ruegg, Markus A.; Brenner, Rolf E.; Ksiazek, Iwona
Author(s) at UniBasel Rüegg, Markus A.
Year 2007
Title Agrin is highly expressed by chondrocytes and is required for normal growth
Journal Histochemistry and Cell Biology
Volume 127
Number 4
Pages / Article-Number 363-74
Keywords agrin, chondrocyte, growth plate, skeletal growth
Mesh terms Aggrecans, metabolism; Agrin, physiology; Animals; Apoptosis, physiology; Cartilage, metabolism; Cell Proliferation; Chickens; Chondrocytes, metabolism; Collagen Type II, metabolism; Female; Gene Expression; Growth Disorders, physiopathology; Growth Plate, metabolism; Immunohistochemistry; Male; Mice; Mice, Knockout; Mice, Transgenic; Receptor Protein-Tyrosine Kinases, metabolism; Reverse Transcriptase Polymerase Chain Reaction
Abstract Agrin is a heparan sulfate proteoglycan that is best known for its crucial involvement in the organization and maintenance of postsynaptic structures at the neuromuscular junction. Consistent with this role, mice deficient of agrin die at birth due to respiratory failure. Here we examined the early postnatal development of agrin-deficient mice in which perinatal death was prevented by transgenic expression of neural agrin in motor neurons. Such transgenic, agrin-deficient mice were born at Mendelian ratio but exhibited severe postnatal growth retardation. Growth plate morpholgy was markedly altered in these mice, with changes being most prominent in the hypertrophic zone. Compression of this zone was not caused by reduced viability of hypertrophic chondrocytes, as no differences in the apoptosis rates could be observed. Furthermore, deposition of the major cartilage matrix components collagen type II and aggrecan was slightly reduced in these mice. Consistent with a role for agrin in skeletal development, we show for the first time that agrin is highly expressed by chondrocytes and localizes to the growth plate in wild-type mice. Our data show that agrin is expressed in cartilage and that it plays a critical role in normal skeletal growth.
Publisher Springer
ISSN/ISBN 0948-6143 ; 1432-119X
edoc-URL http://edoc.unibas.ch/dok/A5258379
Full Text on edoc Available
Digital Object Identifier DOI 10.1007/s00418-006-0258-2
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/17186266
ISI-Number WOS:000244782400002
Document type (ISI) Journal Article
 
   

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