A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 153842
Author(s) Moreno, F.; Fowler, A. V.; Hall, M. N.; Silhavy, T. J.; Zabin, I.; Schwartz, M.
Author(s) at UniBasel Hall, Michael N.
Year 1980
Title A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm
Journal Nature
Volume 286
Number 5771
Pages / Article-Number 356-359
Keywords Amino Acid Sequence; Biological Transport; Carrier Proteins/secretion; Cell Membrane/metabolism; Cytoplasm/metabolism; Escherichia coli/*metabolism; Galactosidases/*secretion; Genetic Engineering; Molecular Weight; Protein Precursors/*secretion; Structure-Activity Relationship; beta-Galactosidase/*secretion
Abstract

Escherichia coli strains have been constructed in which lacZ, the gene for the cytoplasmic enzyme beta-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a beta-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.

Publisher Macmillan
ISSN/ISBN 0028-0836
edoc-URL http://edoc.unibas.ch/dok/A5258223
Full Text on edoc No
Digital Object Identifier DOI 10.1038/286356a0
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/6772962
ISI-Number WOS:A1980KA41300024
Document type (ISI) Journal Article
 
   

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