A mechanism of protein localization: the signal hypothesis and bacteria
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 153840
Author(s) Emr, S. D.; Hall, M. N.; Silhavy, T. J.
Author(s) at UniBasel Hall, Michael N.
Year 1980
Title A mechanism of protein localization: the signal hypothesis and bacteria
Journal The Journal of Cell Biology
Volume 86
Number 3
Pages / Article-Number 701-711
Keywords Bacterial Proteins/genetics/*metabolism/secretion; Bacteriophage lambda/genetics; Cell Compartmentation; Escherichia coli/*metabolism; Lac Operon; Membrane Proteins/metabolism; Protein Precursors/*metabolism; Receptors; Virus/*biosynthesis; Ribosomes/metabolism

We are studying the molecular mechanism of cellular protein localization. The availability of genetic techniques, such as gene fusion in Escherichia coli, has made this problem particularly amenable to study in this prokaryote. We have constructed a variety of strains in which the gene coding for an outer membrane protein is fused to the gene coding for a normally cytoplasmic enzyme, beta-galactosidase. The hybrid proteins produced by such strains retain beta-galactosidase activity; this activity serves as a simple biochemical tag for studying the localization of the outer membrane protein. In addition, we have exploited phenotypes exhibited by certain fusion strains to isolate mutants that are altered in the process of protein export. Genetic and biochemical analyses of such mutants have provided evidence that the molecular mechanism of cellular protein localization is strinkingly similar in both bacteria and animal cells.

Publisher Rockefeller University Press
ISSN/ISBN 0021-9525
edoc-URL http://edoc.unibas.ch/dok/A5258221
Full Text on edoc Available
Digital Object Identifier DOI 10.1083/jcb.86.3.701
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/6447703
ISI-Number WOS:A1980KG12400001
Document type (ISI) Article

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