An internal signal sequence : the asialoglycoprotein receptor membrane anchor

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An internal signal sequence : the asialoglycoprotein receptor membrane anchor

JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)

ID	153705
Author(s)	Spiess, M; Lodish, H F
Author(s) at UniBasel	Spiess, Martin
Year	1986
Title	An internal signal sequence : the asialoglycoprotein receptor membrane anchor
Journal	Cell
Volume	44
Number	1
Pages / Article-Number	177-85
Abstract	The human asialoglycoprotein receptor H1 is anchored in the membrane by a single stretch of 20 hydrophobic amino acids; the hydrophilic amino terminus faces the cytoplasm, and the carboxyl terminus is exoplasmic. We show here that glycosylation and insertion of the asialoglycoprotein receptor into the endoplasmic reticulum membrane is cotranslational and SRP-dependent and occurs without proteolytic cleavage. The membrane-anchor domain is necessary for membrane insertion, since a receptor with the segment deleted is neither inserted nor glycosylated. The segment is also sufficient for membrane insertion, since it will initiate translocation of a carboxy-terminal domain of rat alpha-tubulin across the membrane. We propose that a helical hairpin mechanism of membrane insertion is used both by cleaved amino-terminal and uncleaved internal signal sequences.
Publisher	Cell Press
ISSN/ISBN	0092-8674
edoc-URL	http://edoc.unibas.ch/dok/A5258100
Full Text on edoc	No
Digital Object Identifier DOI	10.1016/0092-8674(86)90496-4
PubMed ID	http://www.ncbi.nlm.nih.gov/pubmed/3753585
ISI-Number	WOS:A1986AYV3400019
Document type (ISI)	Journal Article

11/05/2024

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