An internal signal sequence : the asialoglycoprotein receptor membrane anchor
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
ID 153705
Author(s) Spiess, M; Lodish, H F
Author(s) at UniBasel Spiess, Martin
Year 1986
Title An internal signal sequence : the asialoglycoprotein receptor membrane anchor
Journal Cell
Volume 44
Number 1
Pages / Article-Number 177-85
Abstract The human asialoglycoprotein receptor H1 is anchored in the membrane by a single stretch of 20 hydrophobic amino acids; the hydrophilic amino terminus faces the cytoplasm, and the carboxyl terminus is exoplasmic. We show here that glycosylation and insertion of the asialoglycoprotein receptor into the endoplasmic reticulum membrane is cotranslational and SRP-dependent and occurs without proteolytic cleavage. The membrane-anchor domain is necessary for membrane insertion, since a receptor with the segment deleted is neither inserted nor glycosylated. The segment is also sufficient for membrane insertion, since it will initiate translocation of a carboxy-terminal domain of rat alpha-tubulin across the membrane. We propose that a helical hairpin mechanism of membrane insertion is used both by cleaved amino-terminal and uncleaved internal signal sequences.
Publisher Cell Press
ISSN/ISBN 0092-8674
Full Text on edoc No
Digital Object Identifier DOI 10.1016/0092-8674(86)90496-4
PubMed ID
ISI-Number WOS:A1986AYV3400019
Document type (ISI) Journal Article

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