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RNA-primed DNA synthesis in vitro
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 153622
Author(s) Keller, W.
Author(s) at UniBasel Keller, Walter
Year 1972
Title RNA-primed DNA synthesis in vitro
Journal Proceedings of the National Academy of Sciences of the United States of America
Volume 69
Number 6
Pages / Article-Number 1560-4
Keywords Avian leukosis virus/enzymology; Binding Sites; Carcinoma; Cell Line/enzymology; Centrifugation; Density Gradient; Coliphages; DNA Nucleotidyltransferases; DNA; Single-Stranded; Viral/*chemical synthesis; Escherichia coli/enzymology; Humans; Micrococcus/enzymology; Mouth Neoplasms; Neurospora/enzymology; Nucleic Acid Conformation; Nucleic Acid Hybridization; Protein Binding; RNA Nucleotidyltransferases; *RNA; Viral/chemical synthesis; Templates; Genetic
Abstract In vitro DNA synthesis on single-stranded circular DNA can be initiated by RNA primers. RNA chains are covalently extended by DNA polymerase II from KB cells and DNA polymerase I from Micrococcus luteus, but not by an RNA-dependent DNA polymerase from avian myeloblastosis virus. The reaction product consists of DNA chains with a piece of RNA at their 5'-ends, hydrogen bonded to the template DNA. The primer RNA is linked to the product DNA via a 3':5'-phosphodiester bond, and can be specifically removed by ribonuclease H. The possible role of ribonuclease H in RNA-primed DNA synthesis in vivo is discussed.
Publisher National Academy of Sciences
ISSN/ISBN 0027-8424 ; 1091-6490
URL http://www.pnas.org/content/69/6/1560.full.pdf
edoc-URL http://edoc.unibas.ch/dok/A5258020
Full Text on edoc No
Digital Object Identifier DOI 10.1073/pnas.69.6.1560
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/4338598
ISI-Number WOS:A1972M738900053
Document type (ISI) Journal Article
 
   

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02/05/2024