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Assembly of a processive messenger RNA polyadenylation complex
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 153582
Author(s) Bienroth, S.; Keller, W.; Wahle, E.
Author(s) at UniBasel Keller, Walter
Year 1993
Title Assembly of a processive messenger RNA polyadenylation complex
Journal The EMBO Journal
Volume 12
Number 2
Pages / Article-Number 585-94
Keywords GENE EXPRESSION, POLYADENYLATION, POLY(A) POLYMERASE, PROCESSIVITY, RNA PROCESSING
Abstract Polyadenylation of mRNA precursors by poly(A) polymerase depends on two specificity factors and their recognition sequences. These are cleavage and polyadenylation specificity factor (CPSF), recognizing the polyadenylation signal AAUAAA, and poly(A) binding protein II (PAB II), interacting with the growing poly(A) tail. Their effects are independent of ATP and an RNA 5'-cap. Analysis of RNA-protein interactions by non-denaturing gel electrophoresis shows that CPSF, PAB II and poly(A) polymerase form a quaternary complex with the substrate RNA that transiently stabilizes the binding of poly(A) polymerase to the RNA 3'-end. Only the complex formed from all three proteins is competent for the processive synthesis of a full-length poly(A) tail.
Publisher Nature Publishing Group
ISSN/ISBN 0261-4189 ; 1460-2075
URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC413241/pdf/emboj00074-0212.pdf
edoc-URL http://edoc.unibas.ch/dok/A5257980
Full Text on edoc No
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/8440247
ISI-Number WOS:A1993KL71700022
Document type (ISI) Journal Article
 
   

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