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An adenosine deaminase that generates inosine at the wobble position of tRNAs
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 153555
Author(s) Gerber, A. P.; Keller, W.
Author(s) at UniBasel Keller, Walter
Year 1999
Title An adenosine deaminase that generates inosine at the wobble position of tRNAs
Journal Science
Volume 286
Number 5442
Pages / Article-Number 1146-1149
Keywords Adenosine Deaminase/*chemistry/genetics/*metabolism; Amino Acid Motifs; Amino Acid Sequence; Anticodon/*metabolism; Cytidine Deaminase/chemistry; Dimerization; Evolution; Molecular; Genes; Essential; Fungal; Inosine/*metabolism; Molecular Sequence Data; Mutation; RNA; Fungal/metabolism; Transfer/*metabolism; Saccharomyces cerevisiae/chemistry/*enzymology/genetics; Sequence Alignment
Abstract Several transfer RNAs (tRNAs) contain inosine (I) at the first position of their anticodon (position 34); this modification is thought to enlarge the codon recognition capacity during protein synthesis. The tRNA-specific adenosine deaminase of Saccharomyces cerevisiae that forms I(34) in tRNAs is described. The heterodimeric enzyme consists of two sequence-related subunits (Tad2p/ADAT2 and Tad3p/ADAT3), both of which contain cytidine deaminase (CDA) motifs. Each subunit is encoded by an essential gene (TAD2 and TAD3), indicating that I(34) is an indispensable base modification in elongating tRNAs. These results provide an evolutionary link between the CDA superfamily and RNA-dependent adenosine deaminases (ADARs/ADATs).
Publisher American Association for the Advancement of Science
ISSN/ISBN 0036-8075 ; 1095-9203
edoc-URL http://edoc.unibas.ch/dok/A5257953
Full Text on edoc Restricted
Digital Object Identifier DOI 10.1126/science.286.5442.1146
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/10550050
ISI-Number WOS:000083534200040
Document type (ISI) Article
 
   

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