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A micromethod for separation and identification of digestive enzymes in brush border membrane fragments of single human intestinal biopsies
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 153449
Author(s) Freiburghaus, A U; Hauri, H P; Green, J; Hadorn, B
Author(s) at UniBasel Hauri, Hans-Peter
Year 1978
Title A micromethod for separation and identification of digestive enzymes in brush border membrane fragments of single human intestinal biopsies
Journal Clinica Chimica Acta
Volume 86
Number 3
Pages / Article-Number 227-34
Keywords Cell Membrane/*enzymology; Child; Preschool; Duodenum/enzymology/*ultrastructure; Electrophoresis; Polyacrylamide Gel; Humans; Membrane Proteins/analysis; Methods; Microvilli/*enzymology
Abstract A micromethod for the isolation of brush border membrane fragments from single peroral duodenal biopsies, and their subsequent analysis by polyacrylamide gel electrophoresis is described. The quantity of biopsy material used varied between 5 and 15 mg wet weight, leaving enough mucosa for histological examination. By cutting the gels longitudinally into two halves it was possible to identify several maltases, sucrase, isomaltase and lactase and to correlate these enzymatic activities with distinct co-migrating protein peaks. For alkaline phosphatase and enterokinase this correlation was not possible. This method is suitable for the study on single biopsies of the molecular alterations occurring in the various congenital enzyme deficiencies of the human small intestine.
Publisher Elsevier
ISSN/ISBN 0009-8981
edoc-URL http://edoc.unibas.ch/dok/A5257852
Full Text on edoc No
Digital Object Identifier DOI 10.1016/0009-8981(78)90376-5
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/668114
ISI-Number WOS:A1978FE54000001
Document type (ISI) Article
 
   

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