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Induction of cytochrome P450 by phenobarbital in rat liver visualized by monoclonal antibody immunoelectron microscopy in situ
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 153409
Author(s) Marti, U; Hauri, H P; Meyer, U A
Author(s) at UniBasel Hauri, Hans-Peter
Year 1990
Title Induction of cytochrome P450 by phenobarbital in rat liver visualized by monoclonal antibody immunoelectron microscopy in situ
Journal European journal of cell biology
Volume 52
Number 2
Pages / Article-Number 193-200
Keywords Animals; Antibodies; Monoclonal/biosynthesis; Cytochrome P-450 Enzyme System/*biosynthesis; Electrophoresis; Polyacrylamide Gel; Enzyme Induction/drug effects; Enzyme-Linked Immunosorbent Assay; Immunoblotting; Liver/*enzymology/ultrastructure; Male; Microscopy; Immunoelectron/methods; Phenobarbital/*pharmacology; Rats; Inbred Strains
Abstract A panel of murine monoclonal antibodies was produced against phenobarbital-inducible cytochrome P450-PB of rat liver in order to establish specific immunological tools for studying the induction process in situ by immunoelectron microscopy and in vitro by a novel ELISA. Antibody 573/64 was found to be useful for both approaches. The immunolabeling procedure with protein A-colloidal gold applied to Lowicryl K4M-embedded rat liver revealed the rough ER as the primary site of cytochrome P450-PB induction. This organelle showed the highest labeling density 12 h after administration of phenobarbital while after maximal enzyme induction at day 5 the labeling density was highest in the smooth ER. Maximal increase in cytochrome P450-PB was 21-fold by morphometric analysis and 15-fold by ELISA. In addition, the enzyme apparently does neither recycle through the Golgi apparatus nor is it degraded in lysosomes when maximally induced.
Publisher Elsevier
ISSN/ISBN 0171-9335
edoc-URL http://edoc.unibas.ch/dok/A5257812
Full Text on edoc No
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/2081522
ISI-Number WOS:A1990DX42500003
Document type (ISI) Journal Article
 
   

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