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A reversibly palmitoylated resident protein (p63) of an ER-Golgi intermediate compartment is related to a circulatory shock resuscitation protein
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 153398
Author(s) Schweizer, A; Rohrer, J; Jenö, P; DeMaio, A; Buchman, T G; Hauri, H P
Author(s) at UniBasel Hauri, Hans-Peter
Year 1993
Title A reversibly palmitoylated resident protein (p63) of an ER-Golgi intermediate compartment is related to a circulatory shock resuscitation protein
Journal Journal of cell science
Volume 104 ( Pt 3)
Pages / Article-Number 685-94
Keywords Amino Acid Sequence; Animals; Base Sequence; Brefeldin A; *Cell Compartmentation; Cell Line; Cloning; Molecular; Cyclopentanes/pharmacology; DNA/genetics/isolation & purification; Endoplasmic Reticulum/*chemistry; Golgi Apparatus/*chemistry; Humans; Membrane Proteins/*chemistry/genetics/isolation & Molecular Sequence Data; Palmitic Acids/*chemistry; RNA; Messenger/analysis; Sequence Analysis; DNA; Shock/metabolism
Abstract The recently identified 63 kDa membrane protein, p63, is a resident protein of a membrane network interposed in between rough ER and Golgi apparatus. To characterize p63 at the molecular level a 2.91 kb cDNA encoding p63 has been isolated from a human placenta lambda gt10 cDNA library. Sequence analysis of tryptic peptides prepared from isolated p63 confirmed the identify of the cloned gene. The translated amino acid sequence consists of 601 amino acids (65.8 kDa) with a single putative membrane-spanning region and a N-terminal cytoplasmic domain of 106 amino acids. The human p63 cDNA exhibits a high level of sequence identify to the pig hepatic cDNA 3AL (accession number M27092) whose expression is enhanced after resuscitation from circulatory shock. An additional remarkable feature of p63 is that it becomes reversibly palmitoylated when intracellular protein transport is blocked by the drug brefeldin A. Overexpression of p63 in COS cells led to the development of a striking tubular membrane network in the cytoplasm. This suggests that the protein may be determinant for the structure of the p63 compartment.
Publisher Company of Biologists
ISSN/ISBN 0021-9533
edoc-URL http://edoc.unibas.ch/dok/A5257801
Full Text on edoc No
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/8314870
Document type (ISI) Journal Article
 
   

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