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A neutron diffraction study of the headgroup conformation of phosphatidylglycerol from Escherichia coli membranes
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 153076
Author(s) Mischel, M; Seelig, J; Braganza, L F; Büldt, G
Author(s) at UniBasel Seelig, Joachim
Year 1987
Title A neutron diffraction study of the headgroup conformation of phosphatidylglycerol from Escherichia coli membranes
Journal Chemistry and physics of lipids
Volume 43
Number 4
Pages / Article-Number 237-46
Keywords Cell Membrane/analysis; Escherichia coli/*analysis; Lipid Bilayers; Membrane Lipids/*isolation & purification; Models; Molecular; Molecular Conformation; Neutrons; Phosphatidylglycerols/*isolation & Spectrum Analysis/methods; X-Ray Diffraction/methods
Abstract By using neutron diffraction, the headgroup conformation of purified phosphatidylglycerol from Escherichia coli membranes has been investigated. Measurements at 25 degrees C and 15% relative humidity on oriented multilayers of lipid selectively deuterated at the sn-3-position of the glycerol backbone and of the gamma-position of the glycerol headgroup show that the labels are at a mean distance of 23.0 A and 27.6 A from the centre of the hydrocarbon chain region. This suggests that the negatively charged headgroup is oriented at about 30 degrees to the membrane surface. The orientation of the phosphatidylglycerol headgroup makes the negatively charged phosphate group easily accessible to cations present in the adjacent water layer.
Publisher Elsevier Science
ISSN/ISBN 0009-3084
edoc-URL http://edoc.unibas.ch/dok/A5257495
Full Text on edoc No
Digital Object Identifier DOI 10.1016/0009-3084(87)90020-X
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/3301024
ISI-Number WOS:A1987J377700001
Document type (ISI) Journal Article
 
   

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