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2H-NMR studies on ether lipid-rich bacterial membranes : deuterium order profile of Clostridium butyricum
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 153071
Author(s) Johnston, N C; Goldfine, H; Malthaner, M; Seelig, J
Author(s) at UniBasel Seelig, Joachim
Year 1987
Title 2H-NMR studies on ether lipid-rich bacterial membranes : deuterium order profile of Clostridium butyricum
Journal Biochimica et biophysica acta
Volume 899
Number 2
Pages / Article-Number 302-6
Keywords Cell Membrane/analysis; Clostridium/*analysis; Deuterium; Fatty Acids/analysis; Magnetic Resonance Spectroscopy/methods; Membrane Lipids/*analysis; Phospholipids/analysis; Plasmalogens/*analysis
Abstract Palmitic acid specifically deuterated at different carbon atoms, has been incorporated biosynthetically into the membrane lipids of Clostridium butyricum. The lipids of this organism are rich in plasmalogens and their glycerol acetals and exhibit an unusual fatty acyl and alkenyl chain distribution with saturated chains mainly at the sn-2 position and unsaturated chains at the sn-1 position. The ordering of the deuterated hydrocarbon chains in whole cells was measured with deuterium nuclear magnetic resonance and was compared to the order profiles of isolated cell membranes and membranes formed from the total phospholipid extract. The shape of the order profiles was similar for all three membranes, but the absolute values of the order profiles in whole cells and isolated membranes were lower than those of the liposomal lipids. The order profiles have the same characteristic shape as those found for the lamellar liquid-crystalline phases of synthetic diacylphospholipids.
Publisher Elsevier
ISSN/ISBN 0006-3002
edoc-URL http://edoc.unibas.ch/dok/A5257490
Full Text on edoc No
Digital Object Identifier DOI 10.1016/0005-2736(87)90412-3
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/3580370
ISI-Number WOS:A1987H767500020
Document type (ISI) Journal Article
 
   

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