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Ascorbic acid, a vitamin, is observed by in vivo 13C nuclear magnetic resonance spectroscopy of rat liver
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 153003
Author(s) Küstermann, E; Seelig, J; Künnecke, B
Author(s) at UniBasel Seelig, Joachim
Year 1998
Title Ascorbic acid, a vitamin, is observed by in vivo 13C nuclear magnetic resonance spectroscopy of rat liver
Journal American journal of physiology. Endocrinology and metabolism
Volume 274
Number 1 Pt 1
Pages / Article-Number E65-71
Keywords ascorbic acid turnover, glucose metabolism, isotopomer analysis, spin coupling
Abstract The first in vivo detection of a vitamin with nuclear magnetic resonance (NMR) is reported for mammalian liver. Vitamin C, also known as ascorbic acid, was monitored noninvasively in rat liver by "whole body" 13C NMR spectroscopy at high field after infusion of [1,2-13C2]glucose into anesthetized rats. Generally, the carbon resonances of ascorbic acid overlap with those of other highly abundant cellular metabolites, thus precluding their observation in situ. This problem was resolved by taking advantage of the 13C-13C spin couplings introduced by the two covalently bound 13C nuclei in [1,2-13C2]glucose. During glucose metabolism, [5,6-13C2]ascorbic acid was synthesized, which also exhibited characteristic 13C homonuclear spin couplings. This feature enabled the spectral discrimination of ascorbic acid from overlapping singlet resonances of other metabolites. Quantitative analysis of the spin-coupling patterns provided an estimate of the turnover rate of hepatic ascorbic acid in vivo (1.9 +/- 0.4 nmol.min-1.g-1) and a novel approach toward a better understanding of optimal ascorbic acid requirements in humans. The results obtained in vivo were confirmed with high-resolution proton and 13C NMR spectroscopy of liver extracts.
Publisher American Physiological Society
ISSN/ISBN 0002-9513
edoc-URL http://edoc.unibas.ch/dok/A5257424
Full Text on edoc No
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/9458749
ISI-Number WOS:000071505000010
Document type (ISI) Journal Article
 
   

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