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Contamination risks in work with synthetic peptides: flg22 as an example of a pirate in commercial peptide Preparations
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1528747
Author(s) Mueller, Katharina; Chinchilla, Delphine; Albert, Markus; Jehle, Anna K; Kalbacher, Hubert; Boller, Thomas; Felix, Georg
Author(s) at UniBasel Boller, Thomas
Chinchilla, Delphine
Year 2012
Title Contamination risks in work with synthetic peptides: flg22 as an example of a pirate in commercial peptide Preparations
Journal The plant cell
Volume 24
Number 8
Pages / Article-Number 3193-7
Abstract

The pattern recognition receptor FLAGELLIN SENSING2 (FLS2) renders plant cells responsive to subnanomolar concentrations of flg22, the active epitope of bacterial flagellin. We recently observed that a preparation of the peptide IDL1, a signal known to regulate abscission processes via the receptor kinases HAESA and HAESA-like2, apparently triggered Arabidopsis thaliana cells in an FLS2-dependent manner. However, closer investigation revealed that this activity was due to contamination by a flg22-type peptide, and newly synthesized IDL1 peptide was completely inactive in FLS2 signaling. This raised alert over contamination events occurring in the process of synthesis or handling of peptides. Two recent reports have suggested that FLS2 has further specificities for structurally unrelated peptides derived from CLV3 and from Ax21. We thus scrutinized these peptides for activity in Arabidopsis cells as well. While responding to <1 nM flg22, Arabidopsis cells proved blind even to 100 mu M concentrations of CLV3p and axY(s)22. Our results confirm the exquisite sensitivity and selectivity of FLS2 for flg22. They also show that inadvertent contaminations with flg22-type peptides do occur and can be detected even in trace amounts by FLS2.

Publisher American Society of Plant Biologists
ISSN/ISBN 1040-4651
edoc-URL http://edoc.unibas.ch/dok/A6070629
Full Text on edoc No
Digital Object Identifier DOI 10.1105/tpc.111.093815
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/22923674
ISI-Number WOS:000309536600010
Document type (ISI) Journal Article
 
   

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29/03/2024