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Analysis of the eye developmental pathway in Drosophila using DNA microarrays
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 152860
Author(s) Michaut, Lydia; Flister, Susanne; Neeb, Martin; White, Kevin P; Certa, Ulrich; Gehring, Walter J
Author(s) at UniBasel Gehring, Walter Jakob
Year 2003
Title Analysis of the eye developmental pathway in Drosophila using DNA microarrays
Journal Proceedings of the National Academy of Sciences of the United States of America
Volume 100
Number 7
Pages / Article-Number 4024-9
Keywords Animals; Drosophila Proteins/*genetics; Drosophila melanogaster/*embryology; Embryo; Nonmammalian/physiology; Eye/*embryology; Gene Expression Regulation; Developmental; *Oligonucleotide Array Sequence Analysis/methods
Abstract Pax-6 genes encode evolutionarily conserved transcription factors capable of activating the gene-expression program required to build an eye. When ectopically expressed in Drosophila imaginal discs, Pax-6 genes induce the eye formation on the corresponding appendages of the adult fly. We used two different Drosophila full-genome DNA microarrays to compare gene expression in wild-type leg discs versus leg discs where eyeless, one of the two Drosophila Pax-6 genes, was ectopically expressed. We validated these data by analyzing the endogenous expression of selected genes in eye discs and identified 371 genes that are expressed in the eye imaginal discs and up-regulated when an eye morphogenetic field is ectopically induced in the leg discs. These genes mainly encode transcription factors involved in photoreceptor specification, signal transducers, cell adhesion molecules, and proteins involved in cell division. As expected, genes already known to act downstream of eyeless during eye development were identified, together with a group of genes that were not yet associated with eye formation.
Publisher National Academy of Sciences
ISSN/ISBN 0027-8424
edoc-URL http://edoc.unibas.ch/dok/A5257286
Full Text on edoc No
Digital Object Identifier DOI 10.1073/pnas.0630561100
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/12655063
ISI-Number WOS:000182058400089
Document type (ISI) Journal Article
 
   

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29/03/2024