Argonaute CLIP - A method to identify in vivo targets of miRNAs
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1445117
Author(s) Jaskiewicz, Lukasz; Bilen, Biter; Hausser, Jean; Zavolan, Mihaela
Author(s) at UniBasel Zavolan, Mihaela
Year 2012
Title Argonaute CLIP - A method to identify in vivo targets of miRNAs
Journal Methods
Volume 58
Number 2
Pages / Article-Number 106-12
Keywords Argonaute, Ago, miRNA, microRNA, miRNA targets, CLIP, PAR-CLIP, Computational biology
Abstract

microRNAs are important regulators of gene expression that guide translational repression and degradation of target mRNAs. Only relatively few miRNA targets have been characterized, and computational prediction is hampered by the relatively small number of nucleotides that seem to be involved in target recognition. Argonaute (Ago) crosslinking and immunoprecipitation (CLIP) in combination with next-generation sequencing proved to be a successful method for identifying targets of endogenous cellular miRNAs on a transcriptome-wide scale. Here we review various approaches to Ago CLIP, describe in detail the PAR-CLIP method and provide an outline of the necessary computational analysis for identification of in vivo miRNA binding sites.

Publisher Academic Press
ISSN/ISBN 1046-2023
URL http://www.sciencedirect.com/science/article/pii/S1046202312002460
edoc-URL http://edoc.unibas.ch/dok/A6043855
Full Text on edoc No
Digital Object Identifier DOI 10.1016/j.ymeth.2012.09.006
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/23022257
ISI-Number WOS:000316235300005
Document type (ISI) Article
 
   

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