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Assembly of the Yersinia injectisome: the missing pieces
JournalArticle (Originalarbeit in einer wissenschaftlichen Zeitschrift)
 
ID 1271425
Author(s) Diepold, Andreas; Wiesand, Ulrich; Amstutz, Marlise; Cornelis, Guy R
Author(s) at UniBasel Cornelis, Guy R.
Amstutz, Marlise
Wiesand, Ulrich
Year 2012
Title Assembly of the Yersinia injectisome: the missing pieces
Journal Molecular microbiology
Volume 85
Number 5
Pages / Article-Number 878-92
Abstract

The assembly of the type III secretion injectisome culminates in the formation of the needle. In Yersinia, this step requires not only the needle subunit (YscF), but also the small components YscI, YscO, YscX and YscY. We found that these elements act after the completion of the transmembrane export apparatus. YscX and YscY co-purified with the export apparatus protein YscV, even in the absence of any other protein. YscY-EGFP formed fluorescent spots, suggesting its presence in multiple copies. YscO and YscX were required for export of the early substrates YscF, YscI and YscP, but were only exported themselves after the substrate specificity switch had occurred. Unlike its flagellar homologue FliJ, YscO was not required for the assembly of the ATPase YscN. Finally, we investigated the role of the small proteins in export across the inner membrane. No export of the reporter substrate YscP(1-137) -PhoA into the periplasm was observed in absence of YscI, YscO or YscX, confirming that these proteins are required for export of the first substrates. In contrast, YscP(1-137) -PhoA accumulated in the periplasm in the absence of YscF, suggesting that YscF is not required for the function of the export apparatus, but that its polymerization opens the secretin YscC.

Publisher Blackwell
ISSN/ISBN 0950-382X
edoc-URL http://edoc.unibas.ch/dok/A6008480
Full Text on edoc No
Digital Object Identifier DOI 10.1111/j.1365-2958.2012.08146.x
PubMed ID http://www.ncbi.nlm.nih.gov/pubmed/22788867
ISI-Number WOS:000308089900005
Document type (ISI) Journal Article
 
   

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